Taxonomic Characterizations of Soil DW102 and Its Activity against Fungal Pathogens.

BACKGROUND

spp. are soil bacteria that have commercial value from which numerous secondary metabolites such as antifungal compounds have been derived. There is growing concern that antifungal resistance is on the rise, and soil from various geographical places might produce novel antifungal molecules. The aim of this study was to characterize and identify the actinomycetes strain namely isolate DW102, and to evaluate its antimicrobial activity against nosocomial fungal pathogens.

MATERIALS AND METHODS

isolate DW102 was identified based on morphological, cultural, physiological, and biochemical properties, together with 16S rRNA sequence. Its antifungal activity was determined by agar well-diffusion assays.

RESULTS

The isolate DW102 phenotypic and molecular characterization was identified as DW102 and sequencing results were deposited in GenBank under accession number MK508855. Furthermore, the fermented broth of isolate DW102 inhibited the growth of and .

CONCLUSIONS

Phenotypic, molecular, and phylogenetic analysis of DW102 identified the strain to be . The antifungal assay showed that DW102 fermentation broth was active against both and . Further studies are required to use the isolate DW102 as a promising source for the development of antifungal drugs.