Chenna Deepika, Shastry Shamee, Das Soumya
Department of Immunohematology and Blood Transfusion, Kasturba Medical College of Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Department of Transfusion Medicine, All India Institute of Medical Sciences, Nagpur, India.
Malays J Med Sci. 2021 Feb;28(1):35-40. doi: 10.21315/mjms2021.28.1.5. Epub 2021 Feb 24.
Biomaterials containing platelets have been used to promote healing of ulcers and burns, as well as in implantology and maxillofacial and plastic surgery to achieve wound healing and tissue repair. Commercial devices to prepare autologous biomaterials involve diverse preparation methods that can have high production costs and low yields. Hence, we designed a protocol for preparation of large amounts of autologous platelet-rich fibrin (PRF) glue using conventional processing techniques for blood components.
Autologous whole blood collected 72 h before surgery was processed to prepare platelet concentrates and cryoprecipitate. In a closed system, calcium was added to the cryoprecipitate to release autologous thrombin and generate a firm fibrin clot. The fibrin clot, platelets and calcium were then placed in a conical flask in which a PRF glue formed. The protocol was validated through determination of pre- and post-platelet counts and fibrinogen amounts in the product.
Platelets were recovered with 68% efficiency during the preparation. Essentially no platelets or fibrinogen were found in the supernatant of the PRF glue, suggesting that nearly all had been incorporated in a PRF glue having a relatively large (8 cm × 10 cm) size.
The protocol described here is a cost-effective, simple and closed system that can be used to produce large-size PRF glue to promote repair of major surgical defects.
含血小板的生物材料已被用于促进溃疡和烧伤的愈合，以及在植入学、颌面和整形外科中实现伤口愈合和组织修复。制备自体生物材料的商业设备涉及多种制备方法，这些方法可能成本高且产量低。因此，我们设计了一种方案，使用血液成分的传统加工技术制备大量自体富血小板纤维蛋白（PRF）胶。
对术前72小时采集的自体全血进行处理，以制备血小板浓缩物和冷沉淀。在封闭系统中，向冷沉淀中添加钙以释放自体凝血酶并生成坚固的纤维蛋白凝块。然后将纤维蛋白凝块、血小板和钙置于锥形瓶中，在其中形成PRF胶。通过测定产品中血小板计数和纤维蛋白原含量的前后变化来验证该方案。
制备过程中血小板回收率为68%。在PRF胶的上清液中基本未发现血小板或纤维蛋白原，这表明几乎所有成分都已融入尺寸相对较大（8厘米×10厘米）的PRF胶中。
本文所述方案是一种经济高效、简单且封闭的系统，可用于生产大尺寸PRF胶以促进重大手术缺损的修复。
