On Stephen L W, Zhang Yuwei, Gehring Andrew, Patsekin Valery, Chelikani Venkata, Flint Steve, Wang Haoran, Billington Craig, Fletcher Graham C, Lindsay James, Robinson J Paul
Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand.
Eastern Regional Research Center, Agricultural Research Service, USDA, Wyndmoor, PA, United States.
Front Microbiol. 2021 Feb 17;12:641801. doi: 10.3389/fmicb.2021.641801. eCollection 2021.
Isolation of the pathogens and from foods typically rely on slow (10-21 day) "cold enrichment" protocols before confirmed results are obtained. We describe an approach that yields results in 39 h that combines an alternative enrichment method with culture on a non-selective medium, and subsequent identification of suspect colonies using elastic light scatter (ELS) analysis. A prototype database of ELS profiles from five species and six other bacterial genera found in pork mince was established, and used to compare similar profiles of colonies obtained from enrichment cultures from pork mince samples seeded with representative strains of and The presumptive identification by ELS using computerised or visual analyses of 83/90 colonies in these experiments as the target species was confirmed by partial 16S rDNA sequencing. In addition to seeded cultures, our method recovered two naturally occurring strains. Our results indicate that modified enrichment combined with ELS is a promising new approach for expedited detection of foodborne pathogenic yersiniae.
从食品中分离病原体通常依赖于缓慢的（10 - 21天）“冷增菌”方案，才能获得确认结果。我们描述了一种方法，该方法结合了替代增菌方法和在非选择性培养基上培养，并使用弹性光散射（ELS）分析对可疑菌落进行后续鉴定，可在39小时内得出结果。建立了一个来自猪肉末中发现的5个菌种和其他6个细菌属的ELS图谱的原型数据库，并用于比较从接种了代表性菌株的猪肉末样品增菌培养物中获得的菌落的相似图谱。在这些实验中，通过对83/90个菌落进行计算机化或视觉分析，以ELS进行的初步鉴定作为目标菌种，通过部分16S rDNA测序得到了证实。除了接种培养物外，我们的方法还分离出了两株天然存在的菌株。我们的结果表明，改良增菌结合ELS是一种有前景的新方法，可用于快速检测食源性病原体耶尔森菌。
