Lourkisti Radia, Froelicher Yann, Herbette Stéphane, Morillon Raphael, Giannettini Jean, Berti Liliane, Santini Jérémie
CNRS, Equipe de Biochimie et Biologie Moléculaire du Végétal, UMR 6134 SPE, Université de Corse, Corsica, France.
CIRAD UMR AGAP, Station INRAE, Corsica, France.
Front Plant Sci. 2021 Feb 19;11:615335. doi: 10.3389/fpls.2020.615335. eCollection 2020.
The triploidy has proved to be a powerful approach breeding programs, especially in since seedlessness is one of the main consumer expectations. Citrus plants face numerous abiotic stresses including water deficit, which negatively impact growth and crop yield. In this study, we evaluated the physiological and biochemical responses to water deficit and recovery capacity of new triploid hybrids, in comparison with diploid hybrids, their parents ("Fortune" mandarin and "Ellendale" tangor) and one clementine tree used as reference. The water deficit significantly decreased the relative water content (RWC) and leaf gas exchange ( and ) and it increased the levels of oxidative markers (HO and MDA) and antioxidants. Compared to diploid varieties, triploid hybrids limited water loss by osmotic adjustment as reflected by higher RWC, intrinsic water use efficiency (iWUE ) iWUE and leaf proline levels. These had been associated with an effective thermal dissipation of excess energy (NPQ) and lower oxidative damage. Our results showed that triploidy in citrus enhances the recovery capacity after a water deficit in comparison with diploids due to better carboxylation efficiency, restored water-related parameters and efficient antioxidant system.
三倍体已被证明是育种计划中的一种有效方法，特别是因为无籽是消费者的主要期望之一。柑橘类植物面临着许多非生物胁迫，包括水分亏缺，这对生长和作物产量产生负面影响。在本研究中，我们评估了新的三倍体杂种与二倍体杂种、它们的亲本（“福琼”柑和“埃伦代尔”橘柑）以及一棵用作对照的克莱门氏小柑橘树相比，对水分亏缺的生理和生化反应以及恢复能力。水分亏缺显著降低了相对含水量（RWC）和叶片气体交换（光合速率和气孔导度），并增加了氧化标记物（过氧化氢和丙二醛）和抗氧化剂的水平。与二倍体品种相比，三倍体杂种通过渗透调节限制了水分流失，这表现为较高的相对含水量、内在水分利用效率（iWUE）和叶片脯氨酸水平。这些与过剩能量的有效热耗散（非光化学猝灭）和较低的氧化损伤有关。我们的结果表明，与二倍体相比，柑橘中的三倍体由于更好的羧化效率、恢复的与水分相关的参数和有效的抗氧化系统，增强了水分亏缺后的恢复能力。
