Aguilar-Luis Miguel Angel, Casas Apayco Leslie, Tinco Valdez Carmen, De Lama-Odría María Del Carmen, Weilg Claudia, Mazulis Fernando, Silva-Caso Wilmer Gianfranco, Del Valle-Mendoza Juana Mercedes
School of Medicine, Research and Innovation Center of the Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru.
Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru.
Int J Dent. 2021 Feb 24;2021:2695793. doi: 10.1155/2021/2695793. eCollection 2021.
Severe periodontal disease is highly prevalent worldwide, affecting 20% of the population between the ages of 35 and 44 years. The etiological epidemiology in Peru is scarce, even though some studies describe a prevalence of 48.5% of periodontal disease in the general population. Periodontitis is one of the most prevalent oral diseases associated with site-specific changes in the oral microbiota and it has been associated with a socioeconomic state. This study aimed to determine the etiology and resistance profile of bacteria identified in a group of Peruvian patients with periodontal disease.
Six subgingival plaque samples were collected from eight patients with severe periodontitis. Bacterial identification was carried out by an initial culture, PCR amplification, and subsequently DNA sequencing. We evaluated the antibiotic susceptibility by the disk diffusion method.
Variable diversity in oral microbiota was identified in each one of the eight patients. The bacterial genus most frequently found was spp. (15/48, 31.3%) followed by spp. (11/48, 22.9%), spp. (9/48, 18.8%), and spp. (4/48, 8.3%). The most common species found was (8/48, 16.7%). The antimicrobial susceptibility assay varied according to the species tested; however, among all the isolates evaluated, was resistant to penicillin and tetracycline; was resistant to dicloxacillin; and was resistant to amoxicillin + clavulanic acid and metronidazole but also susceptible to trimethoprim-sulfamethoxazole.
The most prevalent periodontal bacterium found in this study was Specific antimicrobial therapy is required to improve the treatment outcomes of patients with periodontal disease and avoid antibiotic resistance.
重度牙周病在全球范围内高度流行，影响着20%年龄在35至44岁之间的人群。秘鲁的病因流行病学资料匮乏，尽管一些研究表明普通人群中牙周病的患病率为48.5%。牙周炎是与口腔微生物群特定部位变化相关的最常见口腔疾病之一，并且与社会经济状况有关。本研究旨在确定一组患有牙周病的秘鲁患者中鉴定出的细菌的病因及耐药情况。
从8例重度牙周炎患者中采集6份龈下菌斑样本。通过初始培养、PCR扩增以及随后的DNA测序进行细菌鉴定。我们采用纸片扩散法评估抗生素敏感性。
在8例患者中的每一例都鉴定出了口腔微生物群的可变多样性。最常发现的细菌属是 菌属（15/48，31.3%），其次是 菌属（11/48，22.9%）、 菌属（9/48，18.8%）和 菌属（4/48，8.3%）。最常见的菌种是 （8/48，16.7%）。抗菌药物敏感性试验因所测试的菌种而异；然而，在所有评估的分离株中， 对青霉素和四环素耐药； 对双氯西林耐药； 对阿莫西林+克拉维酸和甲硝唑耐药，但对甲氧苄啶-磺胺甲恶唑敏感。
本研究中发现的最常见的牙周细菌是 。需要特定的抗菌治疗来改善牙周病患者的治疗效果并避免抗生素耐药性。
