Colombo Ana Paula V, Boches Susan K, Cotton Sean L, Goodson J Max, Kent Ralph, Haffajee Anne D, Socransky Sigmund S, Hasturk Hatice, Van Dyke Thomas E, Dewhirst Floyd, Paster Bruce J
Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
J Periodontol. 2009 Sep;80(9):1421-32. doi: 10.1902/jop.2009.090185.
This study compared the subgingival microbiota of subjects with refractory periodontitis (RP) to those in subjects with treatable periodontitis (GRs = good responders) or periodontal health (PH) using the Human Oral Microbe Identification Microarray (HOMIM).
At baseline, subgingival plaque samples were taken from 47 subjects with periodontitis and 20 individuals with PH and analyzed for the presence of 300 species by HOMIM. The subjects with periodontitis were classified as having RP (n = 17) based on mean attachment loss (AL) and/or more than three sites with AL >or=2.5 mm after scaling and root planing, surgery, and systemically administered amoxicillin and metronidazole or as GRs (n = 30) based on mean attachment gain and no sites with AL >or=2.5 mm after treatment. Significant differences in taxa among the groups were sought using the Kruskal-Wallis and chi(2) tests.
More species were detected in patients with disease (GR or RP) than in those without disease (PH). Subjects with RP were distinguished from GRs or those with PH by a significantly higher frequency of putative periodontal pathogens, such as Parvimonas micra (previously Peptostreptococcus micros or Micromonas micros), Campylobacter gracilis, Eubacterium nodatum, Selenomonas noxia, Tannerella forsythia (previously T. forsythensis), Porphyromonas gingivalis, Prevotella spp., Treponema spp., and Eikenella corrodens, as well as unusual species (Pseudoramibacter alactolyticus, TM7 spp. oral taxon [OT] 346/356, Bacteroidetes sp. OT 272/274, Solobacterium moorei, Desulfobulbus sp. OT 041, Brevundimonas diminuta, Sphaerocytophaga sp. OT 337, Shuttleworthia satelles, Filifactor alocis, Dialister invisus/pneumosintes, Granulicatella adiacens, Mogibacterium timidum, Veillonella atypica, Mycoplasma salivarium, Synergistes sp. cluster II, and Acidaminococcaceae [G-1] sp. OT 132/150/155/148/135) (P <0.05). Species that were more prevalent in subjects with PH than in patients with periodontitis included Actinomyces sp. OT 170, Actinomyces spp. cluster I, Capnocytophaga sputigena, Cardiobacterium hominis, Haemophilus parainfluenzae, Lautropia mirabilis, Propionibacterium propionicum, Rothia dentocariosa/mucilaginosa, and Streptococcus sanguinis (P <0.05).
As determined by HOMIM, patients with RP presented a distinct microbial profile compared to patients in the GR and PH groups.
本研究使用人类口腔微生物鉴定微阵列（HOMIM），比较了难治性牙周炎（RP）患者与可治疗性牙周炎患者（GRs = 良好反应者）或牙周健康（PH）者的龈下微生物群。
在基线时，从47例牙周炎患者和20例牙周健康个体中采集龈下菌斑样本，通过HOMIM分析300种菌种的存在情况。牙周炎患者根据平均附着丧失（AL）和/或在龈上洁治、根面平整、手术以及全身应用阿莫西林和甲硝唑后，有超过三个位点的AL≥2.5 mm被分类为患有RP（n = 17），或根据平均附着增加以及治疗后无位点的AL≥2.5 mm被分类为GRs（n = 30）。使用Kruskal-Wallis检验和卡方检验寻找各组间分类群的显著差异。
与无疾病（PH）的个体相比，疾病患者（GR或RP）中检测到的菌种更多。RP患者与GRs或牙周健康者的区别在于，推定的牙周病原体频率显著更高，如微小单胞菌（以前的微小消化链球菌或微小微单胞菌）、纤细弯曲菌、结节真杆菌、有害月形单胞菌、福赛坦氏菌（以前的福赛拟杆菌）、牙龈卟啉单胞菌、普氏菌属、密螺旋体属以及腐蚀艾肯菌，还有不常见的菌种（解乳糖假拉米菌、TM7菌种口腔分类单元[OT] 346/356、拟杆菌属OT 272/274、穆尔氏解硫杆菌、脱硫球菌属OT 041、短小短波单胞菌、球形噬细胞菌属OT 337、卫星舒氏菌、具核丝状杆菌、不可见/肺炎戴阿李斯特菌、毗邻颗粒链菌、胆小微杆菌、非典型韦荣球菌、唾液支原体、协同菌属II群以及氨基酸球菌科[G-1]OT 132/150/155/148/135）（P <0.05）。与牙周炎患者相比，在牙周健康个体中更普遍的菌种包括放线菌属OT 170、放线菌属I群、产黑色素普雷沃菌、人心杆菌、副流感嗜血杆菌、奇异劳特罗普菌、丙酸丙酸杆菌、龋齿/粘液罗氏菌以及血链球菌（P <0.05）。
通过HOMIM确定，与GR组和PH组患者相比，RP患者呈现出独特的微生物特征。
