Li Kuikui, Jiang Chaofeng, Tan Haidong, Li Junyan, Xu Yali, Tang Dejian, Zhao Xiaoming, Liu Qishun, Li Jianguo, Yin Heng
Key Laboratory of Se-enriched Products Development and Quality Control, Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Laboratory of Se-enriched Food Development, Ankang, 725000 Shaanxi China.
Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian, 116023 China.
3 Biotech. 2021 Mar;11(3):129. doi: 10.1007/s13205-021-02676-0. Epub 2021 Feb 18.
Konjac glucomannan oligosaccharide has attracted much attention due to its broad biological activities. Specific glucomannan degrading enzymes are effective tools for the production of oligosaccharides from konjac glucomannan. However, there are still few reports of commercial enzymes that can specifically degrade konjac glucomannan. The gene encoding a glucomannanase consisting of 553 amino acids (61.5 kDa) from 3-3 was cloned and heterologous expressed in BL21 (DE3). The recombinant GluB showed high specificity for the degradation of konjac glucomannan. Moreover, the hydrolytic products of GluB degrade konjac glucomannan were a series of oligosaccharides with degrees of polymerisation of 2-12. Furthermore, the biochemical properties indicated that GluB is the optimal active at 45 to 55 °C and pH 5.0-6.0, and shows highly pH stability over a very broad pH range. The present characteristics indicated that GluB is a potential tool to be used to produce oligosaccharides from konjac glucomannan.
魔芋葡甘露聚糖寡糖因其广泛的生物活性而备受关注。特定的葡甘露聚糖降解酶是从魔芋葡甘露聚糖生产寡糖的有效工具。然而，关于能够特异性降解魔芋葡甘露聚糖的商业酶的报道仍然很少。从3-3中克隆了编码由553个氨基酸（61.5 kDa）组成的葡甘露聚糖酶的基因，并在BL21（DE3）中进行了异源表达。重组GluB对魔芋葡甘露聚糖的降解表现出高度特异性。此外，GluB降解魔芋葡甘露聚糖的水解产物是一系列聚合度为2-12的寡糖。此外，生化特性表明，GluB在45至55°C和pH 5.0-6.0时具有最佳活性，并且在非常宽的pH范围内表现出高度的pH稳定性。目前的特性表明，GluB是一种从魔芋葡甘露聚糖生产寡糖的潜在工具。
