Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China.
Front Cell Infect Microbiol. 2021 Feb 19;11:568178. doi: 10.3389/fcimb.2021.568178. eCollection 2021.
Bacterial vaginosis (BV) and its recurrence are most commonly associated with the formation of species biofilm. Probiotics are typically used to treat BV; however, the optimal period of probiotic application in BV treatment remains uncertain. The present study aimed to explore the effects of and on various stages of biofilm formation in species. The biofilm-forming ability of seven strains, including one ATCC 14018 and six clinically isolated species, was determined gentian violet staining assay. Moreover, the sensitivity of the planktonic and biofilm forms toward metronidazole and clindamycin was assessed microdilution broth method. Xbb-LR-1 and Xbb-LC-1 were added during various stages of biofilm formation in species and were cocultured for 24 h. The biofilm thickness of each sample was determined confocal laser scanning microscopy (CLSM). The absolute quantities of species in each sample was obtained real time polymerase chain reaction method, and the pH value was obtained using a pH indicator paper. Biofilm formation by species in a medium with distinct pH values was observed gentian violet staining, CLSM, and scanning electron microscopy (SEM). The biofilm increased the resistance of species toward metronidazole and clindamycin. added at the initial biofilm formation stage in species exhibited highest inhibitory effect, with a percentage inhibition of 38.17% ± 1.35%. When the pH value of the culture medium was <4.5 or >6.5, ATCC 14018 could hardly form a biofilm; however, at pH ≥4.5 and ≤6.5, it was able to form a stronger biofilm. The amount of biofilm attained maximum value at optical density of 3.29 ± 0.28 (595 nm), pH 5.5, and at 36 h. Biofilm formation increases the resistance of species toward antibiotics. Maintaining an acidic vaginal environment with pH <4.5 and a vaginal microbiota dominated by remarkably prevents the formation of species biofilm at the initial stage, which further has a significant impact on the treatment and prevention of biofilm-related infections.
细菌性阴道病（BV）及其复发最常与物种生物膜的形成有关。益生菌通常用于治疗 BV；然而，BV 治疗中益生菌应用的最佳时期仍不确定。本研究旨在探讨 和 对 物种生物膜形成的各个阶段的影响。七种菌株的生物膜形成能力通过结晶紫染色法进行测定，包括一株 ATCC 14018 和六株临床分离的 物种。此外，通过微量稀释肉汤法评估了浮游生物和生物膜形式对甲硝唑和克林霉素的敏感性。在 物种的生物膜形成的各个阶段添加 Xbb-LR-1 和 Xbb-LC-1，并共同培养 24 小时。通过共聚焦激光扫描显微镜（CLSM）测定每个样本的生物膜厚度。通过实时聚合酶链反应法获得每个样本中 物种的绝对数量，并使用 pH 指示剂试纸获得 pH 值。通过结晶紫染色、CLSM 和扫描电子显微镜（SEM）观察具有不同 pH 值的培养基中 物种的生物膜形成。生物膜增加了 物种对甲硝唑和克林霉素的耐药性。在 物种的初始生物膜形成阶段添加 表现出最高的抑制作用，抑制率为 38.17%±1.35%。当培养基的 pH 值<4.5 或>6.5 时，ATCC 14018 几乎不能形成生物膜；然而，当 pH 值≥4.5 且≤6.5 时，它能够形成更强的生物膜。生物膜的数量在吸光度为 3.29±0.28（595nm）、pH5.5 和 36 小时时达到最大值。生物膜形成增加了 物种对抗生素的耐药性。保持 pH<4.5 的酸性阴道环境和以 为主导的阴道微生物群在初始阶段显著阻止 物种生物膜的形成，这对生物膜相关感染的治疗和预防有重要影响。
Zotero 插件