Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China.
Department of Clinical Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China.
Front Cell Infect Microbiol. 2024 May 1;14:1387414. doi: 10.3389/fcimb.2024.1387414. eCollection 2024.
To determine the optimum biofilm formation ratio of () in a mixed culture with ().
ATCC14018, ATCC25922, as well as five strains of were selected from the vaginal sources of patients whose biofilm forming capacity was determined by the Crystal Violet method. The biofilm forming capacity of in anaerobic and non-anaerobic environments were compared using the identical assay. The Crystal Violet method was also used to determine the biofilm forming capacity of a co-culture of and in different ratios. After Live/Dead staining, biofilm thickness was measured using confocal laser scanning microscopy, and biofilm morphology was observed by scanning electron microscopy.
The biofilm forming capacity of under anaerobic environment was similar to that in a 5% CO environment. The biofilm forming capacity of and was stronger at 10:10 CFU/mL than at other ratios (<0.05). Their thicknesses were greater at 10:10 CFU/mL than at the other ratios, with the exception of 10:10 CFU/mL (<0.05), under laser scanning microscopy. Scanning electron microscopy revealed increased biofilm formation at 10:10 CFU/mL and 10:10 CFU/mL, but no discernible was observed at 10:10 CFU/mL.
and showed the greatest biofilm forming capacity at a concentration of 10:10 CFU/mL at 48 hours and could be used to simulate a mixed infection of bacterial vaginosis and aerobic vaginitis .
确定（）与（）混合培养物中生物膜形成的最佳比例。
从患者阴道来源中选择 ATCC14018、ATCC25922 以及 5 株 ，通过结晶紫法测定其生物膜形成能力。使用相同的测定方法比较 在厌氧和非厌氧环境中的生物膜形成能力。还使用结晶紫法测定不同比例的 和 共培养物的生物膜形成能力。使用 Live/Dead 染色后，使用共聚焦激光扫描显微镜测量生物膜厚度，并通过扫描电子显微镜观察生物膜形态。
（）在厌氧环境下的生物膜形成能力与 5% CO 环境下相似。（）和 的生物膜形成能力在 10:10 CFU/mL 时比其他比例更强（<0.05）。在激光扫描显微镜下，它们在 10:10 CFU/mL 时的厚度大于其他比例，除了 10:10 CFU/mL 外（<0.05）。扫描电子显微镜显示在 10:10 CFU/mL 和 10:10 CFU/mL 时生物膜形成增加，但在 10:10 CFU/mL 时未观察到明显的生物膜。
在 48 小时时，浓度为 10:10 CFU/mL 的 和 显示出最大的生物膜形成能力，可用于模拟细菌性阴道病和需氧性阴道炎的混合感染。
