Centre for Inflammation ResearchThe Queen's Medical Research InstituteEdinburgh BioQuarterUniversity of EdinburghEdinburghUnited Kingdom.
MRC Human Genetics UnitInstitute of Genetics and Molecular MedicineUniversity of EdinburghEdinburghUnited Kingdom.
Hepatol Commun. 2020 Nov 13;5(3):358-370. doi: 10.1002/hep4.1628. eCollection 2021 Mar.
The hepatic mesenchyme has been studied extensively in the context of liver fibrosis; however, much less is known regarding the role of mesenchymal cells during liver regeneration. As our knowledge of the cellular and molecular mechanisms driving hepatic regeneration deepens, the key role of the mesenchymal compartment during the regenerative response has been increasingly appreciated. Single-cell genomics approaches have recently uncovered both spatial and functional zonation of the hepatic mesenchyme in homeostasis and following liver injury. Here we discuss how the use of preclinical models, from in vivo mouse models to organoid-based systems, are helping to shape our understanding of the role of the mesenchyme during liver regeneration, and how these approaches should facilitate the precise identification of highly targeted, pro-regenerative therapies for patients with liver disease.
肝脏间充质在肝纤维化的背景下已经得到了广泛研究；然而，在肝脏再生过程中，间充质细胞的作用却知之甚少。随着我们对驱动肝脏再生的细胞和分子机制的认识不断加深，间充质区室在再生反应中的关键作用也越来越受到重视。单细胞基因组学方法最近揭示了肝脏间充质在稳态和肝损伤后的空间和功能分区。在这里，我们讨论了使用临床前模型（从体内小鼠模型到类器官系统）如何帮助我们理解间充质在肝脏再生过程中的作用，以及这些方法应该如何促进针对肝脏疾病患者的高度靶向、促进再生的治疗方法的精确识别。
