Cohen R, Contino M, Hensen D K, Terem T M, Ibsen K I, Granger G A, Wile A G
Department of Surgery, California College of Medicine, Irvine.
Oncology. 1988;45(3):230-5. doi: 10.1159/000226567.
A study was undertaken to enumerate and partially characterize soluble factors generated by tumor-bearing animals capable of suppressing PHA-induced splenocyte proliferation. Sprague-Dawley rats were induced to form malignant ascites by the intraperitoneal injection of the Walker 256 carcinoma. Intact ascites suppressed splenocyte proliferation by 96%. Molecular sieving of the ascites by means of ultrafiltration (10-kilodalton particle cutoff) revealed suppressor activity to reside in both the ultrafiltrate and retentate. Further enumeration of suppressor factors was achieved by preparative polyacrylamide gel electrophoresis of the ascites ultrafiltrate and retentate. Five discrete bands of suppressor activity were resolved in the ultrafiltrate, three of which were heat-labile. Three discrete bands of suppressor activity were resolved in the retentate, none of which were heat-labile. This study underscores the complexity and heterogeneity of soluble factors elaborated in a cancer-bearing animal.
开展了一项研究，以计数并部分表征荷瘤动物产生的能够抑制PHA诱导的脾细胞增殖的可溶性因子。通过腹腔注射Walker 256癌诱导Sprague-Dawley大鼠形成恶性腹水。完整的腹水可将脾细胞增殖抑制96%。通过超滤（截留分子量10千道尔顿颗粒）对腹水进行分子筛分析，发现抑制活性存在于超滤物和截留物中。通过对腹水超滤物和截留物进行制备性聚丙烯酰胺凝胶电泳，进一步对抑制因子进行了计数。在超滤物中分辨出五条离散的抑制活性带，其中三条对热不稳定。在截留物中分辨出三条离散的抑制活性带，其中没有一条对热不稳定。这项研究强调了荷癌动物体内所产生的可溶性因子的复杂性和异质性。
