Wile A G, Cohen R A, Terem T M, Contino M F, Kar R
J Surg Oncol. 1987 Jul;35(3):207-12. doi: 10.1002/jso.2930350315.
Serum-mediated impairment of lymphocyte function was studied in an animal model. Sprague-Dawley rats inoculated intraperitoneally or subcutaneously with the Walker 256 carcinoma underwent sequential sampling of blood. The effect of these serum samples upon phytohemagglutinin-induced blastogenesis of normal rat splenocytes was monitored in an assay utilizing incorporation of (3H)-thymidine. The effect of serum samples from both the subcutaneously and the intraperitoneally inoculated tumor-bearing animals was biphasic. Early on, serum enhanced blastogenesis and later suppressed blastogenesis. Separation of sera into high and low molecular weight components by ultrafiltration demonstrated the enhancing activity to reside in the high molecular weight fraction. The enhancing activity of sera decreased over time from tumor inoculation. Conversely, suppressor activity increased over time from tumor inoculation. This study demonstrated that suppressor activity of sera obtained from animals with advanced tumors is the result of the lack of enhancing activity coupled with the elaboration of suppressor activity.
在一个动物模型中研究了血清介导的淋巴细胞功能损伤。将Walker 256癌腹腔内或皮下接种给Sprague-Dawley大鼠，然后对其进行连续采血。在利用（3H）-胸腺嘧啶掺入的试验中，监测这些血清样本对正常大鼠脾细胞经植物血凝素诱导的细胞增殖的影响。皮下和腹腔内接种荷瘤动物的血清样本的作用是双相的。早期，血清增强细胞增殖，后期则抑制细胞增殖。通过超滤将血清分离成高分子量和低分子量组分，结果表明增强活性存在于高分子量部分。血清的增强活性从肿瘤接种后随时间下降。相反，抑制活性从肿瘤接种后随时间增加。这项研究表明，从晚期肿瘤动物获得的血清的抑制活性是缺乏增强活性并伴有抑制活性产生的结果。
