构建侵染性全长 cDNA 克隆的马铃薯曲叶病毒。

Construction of an infectious full-length cDNA clone of potato aucuba mosaic virus.

机构信息

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

Institute of Alpine Economic Plant, Yunnan Academy of Agricultural Sciences, Lijiang, 674100, Yunnan, People's Republic of China.

出版信息

Arch Virol. 2021 May;166(5):1427-1431. doi: 10.1007/s00705-021-05018-w. Epub 2021 Mar 8.

DOI:10.1007/s00705-021-05018-w

PMID:33682071

Abstract

Potato aucuba mosaic virus (PAMV), a positive single-strand RNA virus, has one of the longest genomes of the viruses in the genus Potexvirus. In 2019, potato samples with mottle and crinkling symptoms from Huzhou, Zhejiang province, China, were identified to be infected with PAMV, potato virus X (PVX), and potato virus Y (PVY) by transcriptome sequencing. To study the effects of single infection by PAMV, the full-length sequence of PAMV from Huzhou (MT193476) was determined and an infectious full-length cDNA clone was constructed. This cDNA clone was infectious by agro-infiltration, leading to systemic symptoms in Nicotiana benthamiana, tomato, pepper, and potato.

摘要

马铃薯喇叭花镶嵌病毒（PAMV）是一种正链单链 RNA 病毒，具有双生病毒属中最长的基因组之一。2019 年，在中国浙江省湖州市出现花叶和皱缩症状的马铃薯样本，通过转录组测序鉴定为感染了马铃薯喇叭花镶嵌病毒、马铃薯 X 病毒（PVX）和马铃薯 Y 病毒（PVY）。为研究 PAMV 单一感染的影响，测定了来自湖州的 PAMV 全长序列（MT193476），并构建了一个可感染的全长 cDNA 克隆。该 cDNA 克隆通过农杆菌浸润具有感染性，导致烟草原生质体、番茄、辣椒和马铃薯出现系统性症状。