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马铃薯奥古巴花叶马铃薯X病毒的全核苷酸序列和基因组结构

The entire nucleotide sequence and genomic organization of potato aucuba mosaic potexvirus.

作者信息

Xu H, Leclerc D, Leung B, AbouHaidar M G

机构信息

Department of Botany, University of Toronto, Ontario, Canada.

出版信息

Arch Virol. 1994;135(3-4):461-9. doi: 10.1007/BF01310031.

Abstract

The entire nucleotide sequence (7057 nucleotides) of cDNA clones of potato aucuba mosaic virus (PAMV) was determined. The genome contains five major putative open reading frames (ORFs), designated from the 5' terminus as encoding putative proteins with M(r) of 187,379 (187 K), 26,326 (26 K), 12,101 (12 K), 8,183 (8 K) and 27,114 (27 K). The genomic organization of PAMV is essentially the same as that of several other potexviruses. The sizes and composition of the proteins encoded by the ORFs are generally similar to those found in other potexviruses. The genome and the capsid protein gene of PAMV, are the largest among the potexviruses sequenced to date.

摘要

测定了马铃薯奥古巴花叶病毒(PAMV)cDNA克隆的完整核苷酸序列(7057个核苷酸)。该基因组包含五个主要的推定开放阅读框(ORF),从5'端起分别编码推定蛋白质,其分子量分别为187379(187K)、26326(26K)、12101(12K)、8183(8K)和27114(27K)。PAMV的基因组结构与其他几种马铃薯X病毒基本相同。ORF编码的蛋白质的大小和组成通常与其他马铃薯X病毒中的相似。PAMV的基因组和衣壳蛋白基因是迄今为止测序的马铃薯X病毒中最大的。

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