Department of Agricultural, Food & Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB, T6G 2P5, Canada.
J Food Sci. 2021 Apr;86(4):1354-1371. doi: 10.1111/1750-3841.15658. Epub 2021 Mar 7.
Wheat (Triticum aestivum) is susceptible to mycotoxin contamination, which can result in significant health risks and economic losses. This research examined the ability of air atmospheric cold plasma (air-ACP) treatment to reduce pure and spiked T-2 and HT-2 mycotoxins' concentration on wheat grains. This study also evaluated the effect of ACP treatment using different gases on wheat grain germination parameters. The T-2 and HT-2 mycotoxin solutions applied on round cover-glass were placed on microscopy slides and wheat grains (0.5 g) were individually spiked with T-2 and HT-2 on their surfaces. Samples were then dried at room temperature (∼24 °C) and treated by air-ACP for 1 to 10 min. Ten minutes of air-ACP treatment significantly reduced pure T-2 and HT-2 concentrations by 63.63% and 51.5%, respectively. For mycotoxin spiked on wheat grains, 10 min air-ACP treatment significantly decreased T-2 and HT-2 concentrations up to 79.8% and 70.4%, respectively. No significant change in the measured quality and color parameters was observed in the ACP-treated samples. Wheat grain germination parameters were not significantly different, when treated with ACP using different gases. Air-ACP treatment and ACP treatment using 80% nitrogen + 20% oxygen improved the germination of wheat grains by 10% and 6%, respectively. This study demonstrated that ACP is an innovative technology with the potential to improve the safety of wheat grains by reducing T-2/HT-2 mycotoxins with an additional advantage of improving their germination. PRACTICAL APPLICATION: Atmospheric cold plasma (ACP) technology has a huge potential to degrade mycotoxins in food grains. This study evaluated the efficacy of ACP to reduce two major mycotoxins (T-2 and HT-2 toxins) in wheat grains. The results of this study will help to develop and scale-up the ACP technology for mycotoxin degradation in grains.
小麦（Triticum aestivum）容易受到真菌毒素污染，这可能导致严重的健康风险和经济损失。本研究考察了空气大气压冷等离子体（air-ACP）处理降低纯和添加 T-2 和 HT-2 真菌毒素在小麦颗粒中浓度的能力。本研究还评估了使用不同气体的 ACP 处理对小麦籽粒发芽参数的影响。将应用于圆形盖玻片的 T-2 和 HT-2 真菌毒素溶液放置在显微镜载玻片上，并将 T-2 和 HT-2 真菌毒素单独喷洒在小麦颗粒（0.5g）表面。然后将样品在室温（约 24°C）下干燥，并通过空气-ACP 处理 1 至 10 分钟。10 分钟的空气-ACP 处理分别显著降低纯 T-2 和 HT-2 浓度 63.63%和 51.5%。对于喷洒在小麦颗粒上的真菌毒素，10 分钟的空气-ACP 处理分别显著降低 T-2 和 HT-2 浓度高达 79.8%和 70.4%。在 ACP 处理的样品中，未观察到测量的质量和颜色参数有显著变化。当使用不同气体的 ACP 处理时，小麦籽粒发芽参数没有显著差异。空气-ACP 处理和 80%氮+20%氧的 ACP 处理分别使小麦籽粒的发芽率提高了 10%和 6%。本研究表明，ACP 是一种具有创新性的技术，具有通过降低 T-2/HT-2 真菌毒素来提高小麦籽粒安全性的潜力，其额外的优势是改善其发芽率。实际应用：大气压冷等离子体（ACP）技术在降解粮食中的真菌毒素方面具有巨大潜力。本研究评估了 ACP 降低小麦粒中两种主要真菌毒素（T-2 和 HT-2 毒素）的功效。本研究的结果将有助于开发和扩大 ACP 技术在谷物中降解真菌毒素的规模。
