Pak J Biol Sci. 2021 Jan;24(1):35-41. doi: 10.3923/pjbs.2021.35.41.
The natural bioactive compounds of Curcuma longa, known as curcuminoids, has been shown to exerts anticancer effects to diverse cancer cell line in vitro, including breast cancer cell line. These curcuminoids consist of curcumin (Cur), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC). Furthermore, there has never been a study to compare the extent of antiproliferative and apoptotic modulation potential between Cur, DMC and BDMC in the breast cancer cell, until now. In the present study, we explore the efficacy among Cur, DMC and BDMC to alters MCF-7 cell viability, which might lead to apoptotic modulation.
This kind of study was performed in vitro whereby the cells were maintained in an appropriate medium and the anticancer effect of curcuminoids (Cur, DMC and BDMC) was measured by using resazurin-based PrestoBlue cell viability assay. Later, MCF-7 breast cancer cells were cultured in 12 wells plate added with different concentrations of Cur, DMC and BDMC for western blotting analysis. Statistical analysis was performed with GraphPad 8, One-way ANOVA and Student's t-test.
The result showed that Cur, DMC and BDMC inhibiting the proliferation of MCF-7 cells. In the concentration dose of 31.25 μg mL-1, the cell viability in cells treated with Cur is 27%, DMC is 31.5% and BDMC is 46%. The IC50 dose of Cur, DMC and BDMC were 25.63, 29.94 and 36.91 μg mL-1.
Cur is more effective in inhibiting proliferation and apoptotic modulation in MCF-7 cells compare to DMC and BDMC. It represents the potential of Cur, DMC and BDMC as adjunctive therapy in treating breast cancer.
姜黄中的天然生物活性化合物，即姜黄素类，已被证明在体外对多种癌细胞系具有抗癌作用，包括乳腺癌细胞系。这些姜黄素类由姜黄素（Cur）、脱甲氧基姜黄素（DMC）和双脱甲氧基姜黄素（BDMC）组成。此外，迄今为止，还没有研究比较 Cur、DMC 和 BDMC 在乳腺癌细胞中抑制增殖和诱导凋亡的潜力。在本研究中，我们探索了 Cur、DMC 和 BDMC 对 MCF-7 细胞活力的影响，这可能导致凋亡的调节。
本研究采用体外方法，将细胞在适当的培养基中培养，并使用基于 Resazurin 的 PrestoBlue 细胞活力测定法来测量姜黄素类（Cur、DMC 和 BDMC）的抗癌作用。随后，将 MCF-7 乳腺癌细胞培养在 12 孔板中，加入不同浓度的 Cur、DMC 和 BDMC 进行 Western blot 分析。统计分析采用 GraphPad 8，单因素方差分析和学生 t 检验。
结果表明，Cur、DMC 和 BDMC 抑制 MCF-7 细胞的增殖。在 31.25μg mL-1 的浓度剂量下，用 Cur 处理的细胞活力为 27%，DMC 为 31.5%，BDMC 为 46%。Cur、DMC 和 BDMC 的 IC50 剂量分别为 25.63、29.94 和 36.91μg mL-1。
与 DMC 和 BDMC 相比，Cur 更能有效抑制 MCF-7 细胞的增殖和诱导凋亡。这代表了 Cur、DMC 和 BDMC 作为治疗乳腺癌的辅助治疗的潜力。
