Crenobacter intestini sp. nov., Isolated from the Intestinal Tract of Konosirus punctatus.

A novel Gram-stain-negative bacterium, designated strain GY 70310, was isolated from the intestinal tract of Konosirus punctatus collected from Minjiang River, China. Cells of the strain were rod-shaped and motile with a single polar flagellum. The result of 16S rRNA gene sequence analyses showed that strain GY 70310 was moderately related to Crenobacter luteus YIM 78141 (94.7%), Paludibacterium paludis KBP-21 (93.8%) and Crenobacter cavernae K1W11S-77 (93.0%). The draft genome of strain GY 70310 consisted of 3.4 Mbp with DNA G+C content of 66.3 mol%, which possessed genes putatively encoding nitrate reductase, nitrite oxidoreductase and urease. The novel strain showed a whole genome average nucleotide identity (OrthoANI) value of 77.1% and a digital DNA-DNA hybridization (dDDH) value of 22.4% with Crenobacter luteus DSM 27258, followed by Crenobacter cavernae K1W11S-77 with OrthoANI and dDDH values of 76.4% and 20.6%, respectively. The major fatty acids (>10%) were identified as summed feature 3 (Cω6c and/or iso-C 2-OH, Cω7c), C and Cω7c. The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified lipid and one unidentified phospholipid. On the basis of phylogenetic analyses, genotypic and chemotaxonomic characteristics, strain GY 70310 represents a novel species of the genus Crenobacter, for which the name Crenobacter intestini sp. nov., is proposed. The type strain is GY 70310 (= CGMCC 1.16821 = KCTC 62945 = NBRC 113900).