Product Development Cell, National Institute of Immunology, New Delhi, India.
Methods Mol Biol. 2021;2279:213-223. doi: 10.1007/978-1-0716-1278-1_17.
Annexin V and propidium iodide staining is widely used for determining the cellular death through apoptosis. In the presence of Ca ions, annexin V has a strong binding affinity for phosphatidylserine, a membrane phospholipid that during apoptosis is translocated from the inner side of the cell membrane to its outer side. On the other hand, propidium iodide has ability for DNA binding and it can only enter into necrotic or late apoptotic cells. This chapter describes a commonly used method for detection of apoptosis in a non-small cell lung cancer cell line using annexin V and propidium iodide dye. We describe the detection of different stages of apoptosis in the A549 lung cancer cell line treated with dihydroartemisinin (DHA). This apoptosis detection method can be used to determine the efficacy of different kinds of drugs on cultured cancer cell lines.
Annexin V 和碘化丙啶染色广泛用于通过凋亡来确定细胞死亡。在钙离子存在的情况下， Annexin V 与磷脂酰丝氨酸具有很强的结合亲和力，磷脂酰丝氨酸是一种膜磷脂，在凋亡过程中从细胞膜的内侧转移到外侧。另一方面，碘化丙啶具有与 DNA 结合的能力，并且只能进入坏死或晚期凋亡细胞。本章描述了一种使用 Annexin V 和碘化丙啶染料检测非小细胞肺癌细胞系中凋亡的常用方法。我们描述了用二氢青蒿素 (DHA) 处理的 A549 肺癌细胞系中不同阶段凋亡的检测。这种凋亡检测方法可用于确定不同类型药物对培养的癌细胞系的疗效。
