Korach K S, Horigome T, Tomooka Y, Yamashita S, Newbold R R, McLachlan J A
Receptor Biology, Section, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Proc Natl Acad Sci U S A. 1988 May;85(10):3334-7. doi: 10.1073/pnas.85.10.3334.
The tissue distribution and levels of estrogen receptor in neonatal mouse uterine tissue were determined in epithelial and stromal fractions separated by mild enzymatic treatment. Proteins of the isolated fractions were separated by gel electrophoresis and receptor was detected on immunoblots with monoclonal antibody H-222. Estrogen receptor protein was detectable in samples of reproductive tract tissue from 5- and 10-day-old mice. The level of receptor in 5-day-old animals was lower per unit DNA in epithelial cells than in stroma. Receptor levels were increased in both tissue types after treatment with diethylstilbestrol, but not with progesterone. Receptor protein present in these neonatal tissues was able to bind steroid as evidenced by affinity labeling with tamoxifen aziridine. Immunohistochemistry on sections of uteri from 4- and 10-day-old mice confirmed the biochemical results and indicated lower nuclear straining in epithelial cells than in stromal cells of uteri of 4-day-old mice. These results demonstrated that estrogen receptor protein is present in both epithelium and stroma of the neonatal mouse uterus, but at a higher level in stromal cells.
通过温和酶处理分离出上皮和基质部分，测定新生小鼠子宫组织中雌激素受体的组织分布和水平。分离部分的蛋白质通过凝胶电泳分离，并用单克隆抗体H-222在免疫印迹上检测受体。在5日龄和10日龄小鼠的生殖道组织样本中可检测到雌激素受体蛋白。5日龄动物上皮细胞中每单位DNA的受体水平低于基质细胞。用己烯雌酚处理后，两种组织类型中的受体水平均升高，但用孕酮处理则无此现象。用他莫昔芬氮丙啶进行亲和标记证明，这些新生组织中存在的受体蛋白能够结合类固醇。对4日龄和10日龄小鼠子宫切片进行的免疫组织化学证实了生化结果，并表明4日龄小鼠子宫上皮细胞中的核染色比基质细胞中的弱。这些结果表明，雌激素受体蛋白存在于新生小鼠子宫的上皮和基质中，但在基质细胞中的水平更高。
