Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 69978 Tel Aviv, Israel; Sagol School of Neuroscience, Tel Aviv University, 69978 Tel Aviv, Israel.
Department of Neuronal Signal Transduction, Max Planck Institute for Neuroscience, Jupiter, FL, 33458, USA.
Curr Opin Neurobiol. 2021 Aug;69:68-75. doi: 10.1016/j.conb.2021.02.002. Epub 2021 Mar 6.
The activity patterns of Individual neurons are highly coordinated and synchronized within neuronal circuits in the brain, much like individual orchestra tools playing together to achieve harmony. Inside neurons, complex protein signaling cascades provide the molecular notes and instructions to each neuron. However, until recently, the dynamic nature of intracellular protein signaling in the intact brain has been eluded. In this review, we focus on recent advancements and the development of approaches to study neuronal signaling dynamics in vivo. We will discuss approaches for the implementation of biosensors for monitoring of protein signaling activities at the levels of individual synapses, dendritic branches, cell-wide neuromodulation, and transcription in the nucleus. Future improvement in these methods and their utilization will undoubtedly yield new insights regarding the intricate link between functional and molecular neuronal dynamics and how they underlie animal's behavior.
大脑中的神经元回路中，单个神经元的活动模式高度协调和同步，就像单个管弦乐队的乐器一起演奏以达到和谐。在神经元内部，复杂的蛋白质信号级联为每个神经元提供分子音符和指令。然而，直到最近，完整大脑内细胞内蛋白质信号的动态性质仍然难以捉摸。在这篇综述中，我们重点介绍了最近的进展以及研究体内神经元信号动力学的方法的发展。我们将讨论用于监测单个突触、树突分支、全细胞神经调制和核中转录水平的蛋白质信号活性的生物传感器的实施方法。这些方法的未来改进及其利用无疑将为功能和分子神经元动力学之间的复杂联系以及它们如何为动物行为提供基础提供新的见解。
