School of Pharmaceutical Sciences, Liaoning University, Shenyang, China.
Department of Biostatistics, College of Public Health and Health Professions & College of Medicine, University of Florida, Gainesville, FL, USA.
Diabetes Metab J. 2021 Mar;45(2):195-208. doi: 10.4093/dmj.2019.0209. Epub 2021 Mar 9.
We hypothesized that specific amino acids or acylcarnitines would have benefits for the differential diagnosis of diabetes. Thus, a targeted metabolomics for amino acids and acylcarnitines in patients with diabetes and its complications was carried out.
A cohort of 54 normal individuals and 156 patients with type 2 diabetes mellitus and/or diabetic complications enrolled from the First Affiliated Hospital of Jinzhou Medical University was studied. The subjects were divided into five main groups: normal individuals, impaired fasting glucose, overt diabetes, diabetic microvascular complications, and diabetic peripheral vascular disease. The technique of tandem mass spectrometry was applied to obtain the plasma metabolite profiles. Metabolomics multivariate statistics were applied for the metabolic data analysis and the differential metabolites determination.
A total of 10 cross-comparisons within diabetes and its complications were designed to explore the differential metabolites. The results demonstrated that eight comparisons existed and yielded significant metabolic differences. A total number of 24 differential metabolites were determined from six selected comparisons, including up-regulated amino acids, down-regulated medium-chain and long-chain acylcarnitines. Altered differential metabolites provided six panels of biomarkers, which were helpful in distinguishing diabetic patients.
Our results demonstrated that the biomarker panels consisted of specific amino acids and acylcarnitines which could reflect the metabolic variations among the different stages of diabetes and might be useful for the differential diagnosis of prediabetes, overt diabetes and diabetic complications.
我们假设特定的氨基酸或酰基辅酶 A 可能对糖尿病的鉴别诊断有益。因此，对糖尿病及其并发症患者的氨基酸和酰基辅酶 A 进行了靶向代谢组学研究。
本研究纳入了来自锦州医科大学第一附属医院的 54 名正常个体和 156 名 2 型糖尿病患者和/或糖尿病并发症患者。将研究对象分为五个主要组：正常个体、空腹血糖受损、显性糖尿病、糖尿病微血管并发症和糖尿病周围血管疾病。采用串联质谱技术获得血浆代谢物图谱。采用代谢组学多元统计方法进行代谢数据分析和差异代谢物的确定。
共设计了 10 个糖尿病及其并发症的交叉比较，以探讨差异代谢物。结果表明，存在 8 个比较，产生了显著的代谢差异。从六个选定的比较中确定了 24 个差异代谢物，包括上调的氨基酸和下调的中链和长链酰基辅酶 A。改变的差异代谢物提供了六个标志物面板，有助于区分糖尿病患者。
我们的结果表明，由特定的氨基酸和酰基辅酶 A 组成的生物标志物组可以反映糖尿病不同阶段的代谢变化，可能有助于对糖尿病前期、显性糖尿病和糖尿病并发症进行鉴别诊断。
