Goerg K J, Wanitschke R, Schwarz M, Meyer zum Büschenfelde K H
1st Medical Clinic, Johannes Gutenberg University, Mainz, FRG.
Pharmacology. 1988;36 Suppl 1:111-9. doi: 10.1159/000138429.
In a short-circuited mucosa-submucosa preparation of the rat descending colon with preserved Meissner's plexus mounted as an everted sac rhein transiently increased the potential difference and short-circuit current (Isc) when administered serosally and mucosally, but serosal application was more effective. Maximal effects were obtained at rhein concentrations of 1.6 X 10(-4) and 3 X 10(-4) mol/l. Net chloride absorption was decreased. Indomethacin (5 X 10(-6) mol/l) did not inhibit the increase of Isc, but omission of calcium from the serosal side as well as tetrodoxin (2 X 10(-7) mol/l) decreased it by 50 and 60%. Mechanical removal of Meissner's plexus partly blocked the effect of rhein, too. It is concluded that rhein stimulates colonic secretion by a direct action on the epithelial cells together with an indirect action via neurohumoral mechanisms.
在保留了迈斯纳神经丛并制成外翻囊的大鼠降结肠短路黏膜 - 黏膜下层标本中，当从浆膜侧和黏膜侧给药时，大黄酸可短暂增加电位差和短路电流（Isc），但从浆膜侧给药更有效。在大黄酸浓度为1.6×10⁻⁴和3×10⁻⁴mol/L时可获得最大效应。净氯吸收减少。吲哚美辛（5×10⁻⁶mol/L）不抑制Isc的增加，但从浆膜侧去除钙以及河豚毒素（2×10⁻⁷mol/L）可使其分别降低50%和60%。机械去除迈斯纳神经丛也部分阻断了大黄酸的作用。结论是，大黄酸通过直接作用于上皮细胞以及经由神经体液机制的间接作用来刺激结肠分泌。
