Chen Chun-Hsien, Wang Yu-Jen, Huang Jian-Ming, Huang Fu-Chin, Lin Wei-Chen
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Department of Parasitology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Antimicrob Agents Chemother. 2023 May 1;65(5). doi: 10.1128/AAC.02066-20. Epub 2021 Mar 8.
spp. are free-living protozoan that cause a serious human eye disease called keratitis (AK). Several new and effective medical therapy for AK patients remains highly debated and therefore, CHG is still considered one of the first lines of treatment for AK patients. We hypothesized that ocular microenvironmental factors are responsible for drug resistance and clinical AK treatment failure. To investigate the influence of the ocular surface on CHG treatment, we tested the effect of several ocular elements on the anti-amoeba activity of CHG. The suspected inhibitory elements, including mucin, albumin, human and amoeba cell lysates, live and heat-killed bacteria, and cornea, were added to the amoebicidal activity platform, where amoeba was incubated with CHG at varying concentrations. Mucin showed a significant inhibitory effect on CHG activity against In contrast, albumin did not affect CHG treatment. Furthermore, human and amoeba cell lysates as well as live and heat-killed bacterial suspensions also significantly inhibited CHG activity. Additionally, we found that pig corneas also reduced CHG activity. In contrast, dry eye drops and their major component, propylene glycol, which is commonly used as eyewash material, did not have an impact on CHG activity. Our results demonstrate the effect of ocular microenvironmental factors on CHG activity and suggest that these factors may play a role in the development of amoeba resistance to CHG and treatment failure.
某些种类是自由生活的原生动物，可导致一种名为棘阿米巴角膜炎（AK）的严重人类眼部疾病。针对AK患者的几种新的有效药物治疗仍存在高度争议，因此，洗必泰（CHG）仍被认为是AK患者的一线治疗方法之一。我们假设眼部微环境因素是导致耐药性和临床AK治疗失败的原因。为了研究眼表对CHG治疗的影响，我们测试了几种眼部成分对CHG抗阿米巴活性的作用。将可疑的抑制成分，包括粘蛋白、白蛋白、人和阿米巴细胞裂解物、活的和热灭活的细菌以及角膜，添加到杀阿米巴活性平台中，在该平台上，阿米巴与不同浓度的CHG一起孵育。粘蛋白对CHG针对（此处原文缺失相关内容）的活性显示出显著的抑制作用。相比之下，白蛋白对CHG治疗没有影响。此外，人和阿米巴细胞裂解物以及活的和热灭活的细菌悬液也显著抑制了CHG的活性。此外，我们发现猪角膜也降低了CHG的活性。相比之下，干眼滴眼液及其主要成分丙二醇（常用作洗眼材料）对CHG活性没有影响。我们的结果证明了眼部微环境因素对CHG活性的影响，并表明这些因素可能在阿米巴对CHG产生耐药性和治疗失败的过程中起作用。
