Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Biosci Biotechnol Biochem. 2021 Apr 24;85(5):1038-1045. doi: 10.1093/bbb/zbab004.
In eukaryotic cells, membrane-surrounded organelles are orchestrally organized spatiotemporally under environmental situations. Among such organelles, vesicular transports and membrane contacts occur to communicate each other, so-called membrane traffic. Filamentous fungal cells are highly polarized and thus membrane traffic is developed to have versatile functions. Early endosome (EE) is an endocytic organelle that dynamically exhibits constant long-range motility through the hyphal cell, which is proven to have physiological roles, such as other organelle distribution and signal transduction. Since filamentous fungal cells are also considered as cell factories, to produce valuable proteins extracellularly, molecular mechanisms of secretory pathway including protein glycosylation have been well investigated. In this review, molecular and physiological aspects of membrane traffic especially related to EE dynamics and protein secretion in filamentous fungi are summarized, and perspectives for application are also described.
在真核细胞中，膜包围的细胞器在环境情况下协调地进行时空组织。在这些细胞器中，囊泡运输和膜接触发生以相互交流，即所谓的膜运输。丝状真菌细胞具有高度极性，因此膜运输得以发展，具有多种功能。早期内体（EE）是一种内吞细胞器，通过菌丝细胞动态地表现出恒定的长程运动，这已被证明具有生理功能，如其他细胞器的分布和信号转导。由于丝状真菌细胞也被认为是细胞工厂，用于在细胞外生产有价值的蛋白质，因此包括蛋白质糖基化在内的分泌途径的分子机制已经得到了很好的研究。在这篇综述中，总结了膜运输的分子和生理方面，特别是与丝状真菌中 EE 动力学和蛋白质分泌相关的方面，并描述了其应用前景。
