Zhang Xin, Cai Xiuhong, Yue Shirui, Chen Zhangxuan, Sun Yulong, Cheng Lei, Xi Yewen, Wang Shunchang
School of Biological Engineering, Huainan Normal University, Huainan 232001, China.
Anhui Huaihe River Basin Center for Aquatic Animal Epidemic Disease Protection and Control, Huainan 232038, China.
Animals (Basel). 2025 Jul 21;15(14):2150. doi: 10.3390/ani15142150.
Water temperature significantly affects the physiological balance of aquatic organisms like crustaceans, and heat shock proteins (HSPs) are crucial for stress resistance and pathogen defense. This study conducted a genome-wide analysis to explore the functional characteristics of the gene family in . Fifteen family members were identified, with several genes showing upregulation under non-lethal heat shock (NLHS) and pathogen challenges. RNA-Seq and qPCR analyses confirmed increased expression of certain during NLHS, indicating NLHS activation of the family to enhance immune regulation. dsRNA-mediated silencing of led to downregulation of TLR pathway genes (e.g., , ), suggesting regulates the TLR signaling pathway for immune responses. These findings reveal that NLHS-induced upregulation improves pathogen resistance, offering insights for addressing temperature fluctuations and disease outbreaks in aquaculture to optimize management practices.
水温显著影响甲壳类等水生生物的生理平衡，热休克蛋白（HSPs）对于抗逆性和病原体防御至关重要。本研究进行了全基因组分析，以探索该基因家族在……中的功能特性。鉴定出了15个该家族成员，有几个基因在非致死性热休克（NLHS）和病原体挑战下表现出上调。RNA测序和定量聚合酶链反应分析证实了在NLHS期间某些……的表达增加，表明NLHS激活该家族以增强免疫调节。双链RNA介导的……沉默导致Toll样受体（TLR）途径基因（如……、……）下调，表明……调节TLR信号通路以进行免疫反应。这些发现揭示了NLHS诱导的……上调可提高病原体抗性，为应对水产养殖中的温度波动和疾病爆发以优化管理实践提供了见解。
