Mechanical Engineering Department, MicroTech Laboratory, Universitat Politècnica de Catalunya, 08222, Terrassa, Barcelona, Spain.
Faculty of mechanical and energy engineering, Shahid Beheshti University, Tehran, 16765-1719, Iran.
Anal Bioanal Chem. 2021 May;413(11):2867-2878. doi: 10.1007/s00216-021-03190-1. Epub 2021 Mar 8.
Blood plasma separation from undiluted blood is an essential step in many diagnostic procedures. This study focuses on the numerical optimization of the microfluidic blood plasma separator (BPS) and experimental validation of the results to achieve portable blood plasma separation with high purity and reasonable yield. The proposed design has two parts: a microchannel for blood processing and a tank below the aforementioned main channel for plasma collection. The study uses 3D computational fluid dynamic analysis to investigate the optimal ratio of heights between the top microchannel and the tank and their geometry at various flow rates. Thereafter, the results are compared with the experimental findings of the fabricated devices. These results are contrasted with some recent reported works to verify the proposed device's contribution to the improvement in the quality and quantity of the extracted plasma. The optimized design is capable of achieving a 19% yield with purity of 77.1%, depending on the requirement of the point-of-care (POC) application. These amounts could be tuned, for instance to 100% pure plasma, but the yield would decrease to 9%. In this study, the candidate application is hemostasis; therefore, the BPS is integrated to a biomimetic surface for hemostasis evaluation near the patients.
从未稀释的血液中分离血浆是许多诊断程序的重要步骤。本研究专注于微流控血浆分离器（BPS）的数值优化，并对结果进行实验验证，以实现具有高纯度和合理产量的便携式血浆分离。所提出的设计有两部分：用于血液处理的微通道和位于上述主通道下方的用于收集血浆的槽。该研究使用 3D 计算流体动力学分析来研究在不同流速下顶部微通道和槽之间的高度最佳比例及其几何形状。然后，将结果与制造设备的实验结果进行比较。将这些结果与一些最近报道的工作进行对比，以验证所提出的设备在提高提取血浆的质量和数量方面的贡献。优化设计能够实现 19%的产率和 77.1%的纯度，具体取决于即时护理（POC）应用的要求。这些数量可以进行调整，例如达到 100%的纯血浆，但产量会下降到 9%。在本研究中，候选应用是止血；因此，BPS 被集成到仿生表面上，以便在患者附近进行止血评估。
