Fu Hao, You ShiQuan, Zhao Dan, An Quan, Zhang Jiachan, Wang Changtao, Wang Dongdong, Li Meng
Beijing Key Lab of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, China.
J Cosmet Dermatol. 2021 Dec;20(12):4052-4059. doi: 10.1111/jocd.14051. Epub 2021 Mar 25.
UVA radiation can cause skin oxidative stress. Tremella fuciformis polysaccharides (TFPS) are the main active ingredient in Tremella. Previous studies have shown that TFPS have protective effects on UVA-induced oxidative stress cells, but the specific protective mechanism has not been clarified.
This study aims to explore the potential protective mechanism of Tremella fuciformis polysaccharides on UVA-induced damage to human dermal fibroblasts cells.
We evaluated the protective ability of TFPS against UVA-induced damage by detecting cell survival rate, the content of reactive oxygen species (ROS) and malondialdehyde (MDA) in the cells, and the scavenging activity of ABTS free radicals, as well as the enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), and the Nrf2-Keap-1 pathway protein and gene were measured to study the protective mechanism of TFPS from photodamage.
TFPS pretreatment can reduce the oxidative stress of UVA-treated human dermal fibroblasts cells. After TFPS pretreatment, the content of ROS and MDA in the cell decreased significantly and the total antioxidant activity was increased. Among them, the active of CAT, SOD, and GSH-Px in the cells increased significantly. TFPS can also protect fibroblasts by up-regulating Nrf2 and down-regulating Keap1 expression. Finally, it was also found that TFPS pretreatment increased the content of collagen I, elastin, and hyaluronic acid (HA) in skin fibroblasts treated with UVA.
These results indicate that a certain concentration of TFPS can effectively alleviate skin damage caused by UVA, and they may be used as an effective component of cosmetics.
紫外线A辐射可导致皮肤氧化应激。银耳多糖（TFPS）是银耳的主要活性成分。先前的研究表明，TFPS对紫外线A诱导的氧化应激细胞具有保护作用，但具体的保护机制尚未阐明。
本研究旨在探讨银耳多糖对紫外线A诱导的人皮肤成纤维细胞损伤的潜在保护机制。
通过检测细胞存活率、细胞内活性氧（ROS）和丙二醛（MDA）含量、ABTS自由基清除活性以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的酶活性，评估TFPS对紫外线A诱导损伤的保护能力，并检测Nrf2-Keap-1通路蛋白和基因，以研究TFPS的光损伤保护机制。
TFPS预处理可降低紫外线A处理的人皮肤成纤维细胞的氧化应激。TFPS预处理后，细胞内ROS和MDA含量显著降低，总抗氧化活性增加。其中，细胞内CAT、SOD和GSH-Px活性显著增加。TFPS还可通过上调Nrf2和下调Keap1表达来保护成纤维细胞。最后，还发现TFPS预处理增加了紫外线A处理的皮肤成纤维细胞中I型胶原蛋白、弹性蛋白和透明质酸（HA）的含量。
这些结果表明，一定浓度的TFPS可有效减轻紫外线A引起的皮肤损伤，它们可能用作化妆品的有效成分。
