College of Pharmacy, Zunyi Medical University, Zunyi, 563000, China.
College of Pharmacy, Zunyi Medical University, Zunyi, 563000, China; Joint International Research Laboratory of Ethnomedicine of Chinese Ministry of Education, Zunyi Medical University, Zunyi, 563000, China.
J Ethnopharmacol. 2022 Nov 15;298:115590. doi: 10.1016/j.jep.2022.115590. Epub 2022 Aug 13.
Dendrobium nobile Lindl. is an orchid species that is found throughout Asia, including Thailand, Laos, Vietnam, and China. It has been used to treat tumors, hyperglycemia, hyperlipidemia, and neurological disorders caused by aging in recent decades.
To investigate the antagonistic effect of Dendrobium nobile Lindl. Polysaccharides (DNLP) on UVA-induced photoaging of Human foreskin fibroblasts (HFF-1) and explore its possible anti-aging mechanisms.
An in vitro photoaging model of dermal fibroblasts was established with multiple UVA irradiations. Fibroblasts were treated with 0.06 mg/ml, 0.18 mg/ml, 0.54 mg/ml of DNLP one day before photodamage induction. The levels of reactive oxygen species (ROS), Malondialdehyde (MDA), cell viability and longevity, Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione peroxidase (GSH-Px) enzymatic activities were determined. We examined how DNLP ameliorates the effects of photoaging, the JNK/c-Fos/c-Jun pathway, senescence-associated β-galactosidase (SA-β-Gal), and MMP expression levels were measured.
UVA irradiation reduced the viability, lifespan, and proliferation of HFF-1 cells, increased ROS and lipid peroxidation and decreased the activities of free radical scavenging enzyme systems SOD, CAT, and GSH-Px. DNLP treatment can reverse UVA damage, reduce SA-β-Gal expression, reduce phosphorylation activation of the JNK/c-Fos/c-Jun pathway and inhibit MMP-1, MMP-2 MMP-3, and MMP-9 protein expression.
DNLP can effectively inhibit UVA damage to HFF-1 and prevent cell senescence. Its mechanism of action may increase antioxidant enzyme activity while inhibiting JNK pathway activation and MMPs expression.
铁皮石斛是一种兰花物种，分布于亚洲各地，包括泰国、老挝、越南和中国。近几十年来，它已被用于治疗肿瘤、高血糖、高血脂和衰老引起的神经紊乱。
研究铁皮石斛多糖（DNLP）对 UVA 诱导的人包皮成纤维细胞（HFF-1）光老化的拮抗作用，并探讨其可能的抗衰老机制。
采用多次 UVA 照射建立皮肤成纤维细胞体外光老化模型。在光损伤诱导前一天，用 0.06mg/ml、0.18mg/ml、0.54mg/ml 的 DNLP 处理成纤维细胞。测定活性氧（ROS）、丙二醛（MDA）、细胞活力和寿命、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）酶活性。检测 DNLP 如何改善光老化的影响，JNK/c-Fos/c-Jun 通路、衰老相关β-半乳糖苷酶（SA-β-Gal）和 MMP 表达水平。
UVA 照射降低了 HFF-1 细胞的活力、寿命和增殖能力，增加了 ROS 和脂质过氧化，降低了自由基清除酶系统 SOD、CAT 和 GSH-Px 的活性。DNLP 处理可逆转 UVA 损伤，降低 SA-β-Gal 表达，降低 JNK/c-Fos/c-Jun 通路磷酸化激活，抑制 MMP-1、MMP-2、MMP-3 和 MMP-9 蛋白表达。
DNLP 能有效抑制 UVA 对 HFF-1 的损伤，防止细胞衰老。其作用机制可能是通过增加抗氧化酶活性，同时抑制 JNK 通路的激活和 MMPs 的表达。
