Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Trivandrum, India.
Academy of Scientific and Innovative Research (AcSIR), CSIR, Ghaziabad, India.
Prep Biochem Biotechnol. 2021;51(10):1026-1035. doi: 10.1080/10826068.2021.1894441. Epub 2021 Mar 9.
Bimetallic or alloy nanoparticles (NPs) have improved properties compared to their monometallic forms. Microalgae being rich in biocompatible reductants and being ecofriendly are potential sources to synthesize fuctionalized NPs. In this study, biosynthesis of silver, gold, and bimetallic NPs was carried out bioreduction using aqueous extract of algal isolate , inhabitant of non-arable land. is known to contain highly bioactive functional moieties, which can serve as nanobiofactories for metallic NPs. Various characterization techniques UV-visible spectrophotometer, X-ray diffraction analysis, X-ray photo-electron spectroscopy, and Raman spectroscopy were employed to determine their composition, structure, and crystal phase. The monometallic and bimetallic particles were found to be crystalline state and generally in a spherical shape. Their size ranged from 5 to 45 nm and the corresponding FTIR spectra indicated that the specific organic functional groups from algal extract were involved in the bio-reduction. Furthermore, the core-shell in the case of Au-Ag NPs was formed due to the simultaneous reduction of gold and silver ions. An enhanced and more pronounced Raman spectra of Au-Ag NP compared to individual Au NP indicated the improved properties of bimetallic NPs, the latter having been of immense potential to be used as sensors in industries.
双金属或合金纳米粒子（NPs）比其单金属形式具有更好的性能。微藻富含生物相容性还原剂，且对环境友好，是合成功能化 NPs 的潜在来源。在这项研究中，使用非耕地居民藻类分离物的水提物进行生物还原，合成了银、金和双金属 NPs。已知该水提物含有高度生物活性的功能基团，可作为金属 NPs 的纳米生物工厂。采用各种表征技术，如紫外-可见分光光度计、X 射线衍射分析、X 射线光电子能谱和拉曼光谱，来确定它们的组成、结构和晶体相。单金属和双金属颗粒被发现处于结晶状态，通常呈球形。它们的尺寸范围为 5 至 45nm，相应的傅里叶变换红外光谱表明，藻类提取物中的特定有机功能基团参与了生物还原。此外，在 Au-Ag NPs 的情况下，由于金和银离子的同时还原，形成了核壳结构。与单个 Au NP 相比，Au-Ag NP 的增强和更明显的拉曼光谱表明双金属 NPs 的性能得到了改善，后者具有作为工业传感器的巨大潜力。
