de Saint-Georges L, Van Gorp U, Maisin J R
Centre d'Etude de l'Energie Nucléaire, Department of Biology, Mol, Belgium.
Scanning Microsc. 1988 Mar;2(1):537-43.
Male mice of the Balb/c strain were exposed, at an age of three months, to a single dose of 10 or 20 Gy on the right hemithorax. At 3, 4, 6, 9 and 12 months after exposure, lungs were processed for electron microscopy following a standardized procedure in order to allow stereological analysis. By this method, the arithmetical mean thickness and, the air-blood barrier mean thickness in the lung parenchyma was shown to increase quickly with time by oedemization and fibrinization of the septal space. The ratio endothelium/epithelium surfaces (SI/SE) gradually decreased by reduction of both surfaces but this was more marked for Si. The endothelium and epithelium were both highly damaged. Quantitative results indicate that damage to the epithelial cells and mainly to type II, appear at the same time as damage to the endothelium. From the time lapse quantitation it is not possible to determine which one plays the predominant role in the radiation pneumonitis. The strong reaction of the basement membrane and mainly of the interstitial cells could play a decisive role in the evolution of the illness.
将三个月大的Balb/c品系雄性小鼠的右半胸暴露于10或20 Gy的单次剂量辐射下。在暴露后3、4、6、9和12个月，按照标准化程序对肺进行处理以进行电子显微镜检查，以便进行体视学分析。通过这种方法，肺实质中的算术平均厚度和气-血屏障平均厚度显示随着时间的推移，通过间隔空间的水肿和纤维蛋白化而迅速增加。内皮/上皮表面比率（SI/SE）由于两个表面的减少而逐渐降低，但对Si来说更为明显。内皮和上皮均受到高度损伤。定量结果表明，上皮细胞尤其是II型上皮细胞的损伤与内皮损伤同时出现。从时间推移定量分析中无法确定哪一个在放射性肺炎中起主要作用。基底膜尤其是间质细胞的强烈反应可能在疾病的发展中起决定性作用。
