College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, China.
College of Animal Science & Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, China.
Transbound Emerg Dis. 2022 May;69(3):1046-1055. doi: 10.1111/tbed.14060. Epub 2021 Mar 31.
Astroviruses are a non-enveloped virus with large host range breadth. AstV-associated gastroenteritis in human and animal, nephritis in chicken, gout in gosling and hepatitis in duckling pose great threats to public health and poultry industry. Since early 2020, continuous emergence of fatal goose astrovirus (GAstV) infections characterized by articular and visceral gout was reported in China. Here, we described two outbreaks of emerging gout disease in two different goose farms of central China. Two virulent GAstV strains, designated as HNKF-1/China/2020 and HNSQ-6/China/2020, were isolated, and the fifth passage of the isolates could cause urate crystals accumulated in the allantoic fluid and even deposited around great vessels and embryo bodies. Meanwhile, the source of these GAstV outbreaks was tracked to goose hatcheries. The prevalence of GAstV in the goose embryos with hatch failure was confirmed, and embryo-origin HNXX-6/China/2020 was further isolated. The complete genome of these three newly isolates was then sequenced and analysed. The results showed that Chinese GAstVs have formed two distinct groups, and the three GAstV isolates, as well as most of the Chinese GAstVs, belong to the G-I group. There are several amino acid mutations in the three newly identified GAstVs, such as A520T, S535R, V555I and A782T in ORF1a and Q229P in ORF2, suggesting the field stains, HNKF-1/China/2020 and HNSQ-6/China/2020, might derive from the weak goose embryo via vertical transmission. Moreover, the phylogenetic analysis of the complete viral genome and individual viral proteins revealed that Chinese GAstV strains have been constantly evolving towards more complicated and various directions. Our study reported the recently emerging GAstV outbreaks in central China, and further analysed the genetic characteristics of three virulent G-I GAstV isolates from commercial goose farms and goose hatchery, indicating the diverse transmission of the virus and providing a basis for developing effective preventive measures and control strategies.
星状病毒是一种无包膜病毒，宿主范围广泛。人类和动物的星状病毒相关性胃肠炎、鸡的肾炎、鹅的痛风和鸭的肝炎对公共卫生和家禽业构成了巨大威胁。自 2020 年初以来，中国连续报告了以关节和内脏痛风为特征的致命鹅星状病毒（GAstV）感染。本研究描述了中国中部两个不同鹅场新兴痛风病的两次暴发。分离到两株强毒 GAstV 株，分别命名为 HNKF-1/中国/2020 和 HNSQ-6/中国/2020，第五代分离株可导致尿酸盐晶体在尿囊液中积累，甚至沉积在大血管和胚胎体周围。同时，这些 GAstV 暴发的源头追溯到鹅孵化场。证实了孵化失败的鹅胚胎中 GAstV 的流行，并进一步分离出胚胎源 HNXX-6/中国/2020。然后对这三个新分离株的全基因组进行测序和分析。结果表明，中国 GAstV 已形成两个不同的组，这三个 GAstV 分离株以及大多数中国 GAstV 属于 G-I 组。在这三个新鉴定的 GAstV 中，有几个氨基酸突变，如 ORF1a 中的 A520T、S535R、V555I 和 A782T 以及 ORF2 中的 Q229P，表明 HNKF-1/中国/2020 和 HNSQ-6/中国/2020 这两个分离株可能是通过垂直传播从弱鹅胚中产生的。此外，完整病毒基因组和单个病毒蛋白的系统进化分析表明，中国 GAstV 株一直在向更复杂和多样化的方向进化。本研究报道了中国中部最近暴发的 GAstV 疫情，并进一步分析了来自商业鹅场和鹅孵化场的三个强毒 G-I GAstV 分离株的遗传特征，表明病毒的传播方式多样，为制定有效的预防和控制策略提供了依据。
