Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, 530004, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, 530004, China; Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, 530004, China.
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Microb Pathog. 2024 Sep;194:106829. doi: 10.1016/j.micpath.2024.106829. Epub 2024 Jul 30.
Goose astroviruses (GAstVs) are important pathogens which can cause gout in goslings leading to huge economic losses for the goose farming industry in China. In 2023, an infectious disease characterized by visceral gout broke out in commercial goose farms in Guangxi and Guangdong provinces of China. In this study, two GAstV strains of GXNN and GDCS were successfully isolated from these two disease-ridden goose farms. The complete genomic lengths of these two strains were 7166 bp, and phylogenetic analysis showed that they were both GAstV-2 subtypes. The 3-dimensional structures of the capsid protein were predicted and six characteristic mutation sites at amino acid positions 60, 61, 228, 229, 456 and 523 were found within the strong antigenic regions. A recombination event occurred at 6833-7070 nt between the GAstV TZ03 and Turkey astrovirus CA/00 and this was detected in both the GXNN and GDCS strains. Another recombinant event occurred at 63-2747 nt between the GAstV XT1 and GAstV SDPY and this was detected in the GDCS strain. When 1-day-old goslings were infected with the novel GXNN and GDCS strains, they showed severe visceral gout. This was accompanied by enlarged spleens, liver hemorrhages and urate deposits in the kidneys and ureters and their blood urea nitrogen levels were significantly elevated. The mortality rates of the GXNN- and GDCS-infected groups were pathogenically high at 80 % and 60 %, respectively. These results will promote our understanding of the evolution and epidemic potential of GAstVs in China.
鹅星状病毒（GAstVs）是重要的病原体，可引起雏鹅痛风，给中国养鹅业造成巨大经济损失。2023 年，中国广西和广东省的商业鹅养殖场爆发了一种以内脏痛风为特征的传染病。本研究从这两个发病鹅场成功分离到两株 GAstV 株 GXNN 和 GDCS。这两株病毒的完整基因组长度均为 7166bp，系统进化分析表明它们均属于 GAstV-2 亚型。预测了衣壳蛋白的三维结构，在强抗原区域的氨基酸位置 60、61、228、229、456 和 523 发现了六个特征性突变位点。在 GAstV TZ03 和土耳其星状病毒 CA/00 之间的 6833-7070nt 发生了重组事件，在 GXNN 和 GDCS 株中均检测到该事件。在 GAstV XT1 和 GAstV SDPY 之间的 63-2747nt 也发生了另一次重组事件，在 GDCS 株中检测到该事件。当 1 日龄雏鹅感染新型 GXNN 和 GDCS 株时，表现出严重的内脏痛风。同时伴有脾脏肿大、肝脏出血和肾脏及输尿管尿酸沉积，其血尿素氮水平显著升高。感染 GXNN 和 GDCS 的鹅死亡率分别高达 80%和 60%。这些结果将促进我们对中国 GAstVs 进化和流行潜力的理解。
