Pulmonary Research Institute at LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany.
LungenClinic Grosshansdorf, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Grosshansdorf, Germany.
Int J Chron Obstruct Pulmon Dis. 2021 Mar 1;16:487-493. doi: 10.2147/COPD.S284938. eCollection 2021.
We recently introduced a sputum cell quality score to rate how cell morphology, cellular debris and squamous cell contamination influence inflammatory cell identification during microscopic evaluation. However, sputum cell quality is generally not considered for the interpretation of sputum fluid phase biomarkers. Therefore, we compared the soluble protein concentrations between sputum samples with different cell quality. The impact of cell quality was compared to other factors potentially affecting soluble biomarker concentrations.
A comprehensive sputum dataset from 154 clinically stable COPD patients was used to analyse the differences and the variability of sputum supernatant concentrations for 23 proteins between low, medium, and high sputum cell quality samples. A model was developed and tested to compare the impact of different factors on sputum supernatant protein levels.
Mean percentages of sputum macrophages, neutrophils, eosinophils, monocytes and lymphocytes showed no significant differences between low, medium and high cell quality levels. The mean percentage of squamous cells were lower, while total cell count/mL sputum and cell viability were significantly higher in sputum samples with higher cell quality. The concentrations of Interleukin-6, Interleukin-8 and Tumor Necrosis Factor Receptor 2 were significantly increased in sputum samples of higher cell quality. The variability of most protein concentrations declined with increasing cell quality levels. Sixteen proteins showed significantly negative correlations with the percentage of squamous cells. For 14 proteins we observed a positive correlation with cell number/mL sputum. Multiple regression analysis shows that generally less than 30% of the protein variability can be explained by the included factors.
Sputum cell quality has a significant impact on some soluble biomarker concentrations in sputum supernatant. Sputum samples with low sputum cell quality show a higher variability of fluid phase proteins in comparison to medium and high sputum cell quality levels.
我们最近引入了一个痰细胞质量评分，以评估细胞形态、细胞碎片和鳞状细胞污染如何影响显微镜评估过程中炎症细胞的识别。然而，痰细胞质量通常不被用于解释痰液相生物标志物。因此，我们比较了不同细胞质量的痰样本之间的可溶性蛋白浓度。将细胞质量的影响与其他可能影响可溶性生物标志物浓度的因素进行了比较。
使用来自 154 例临床稳定 COPD 患者的综合痰数据集，分析了低、中、高细胞质量样本中 23 种蛋白质的痰上清液浓度之间的差异和变异性。建立并测试了一个模型，以比较不同因素对痰上清液蛋白水平的影响。
低、中、高细胞质量水平的痰巨噬细胞、中性粒细胞、嗜酸性粒细胞、单核细胞和淋巴细胞的平均百分比无显著差异。高细胞质量的痰样本中鳞状细胞的平均百分比较低，而总细胞计数/ml 痰和细胞活力明显较高。细胞质量较高的痰样本中白细胞介素-6、白细胞介素-8 和肿瘤坏死因子受体 2 的浓度显著升高。随着细胞质量水平的提高，大多数蛋白质浓度的变异性下降。16 种蛋白质与鳞状细胞百分比呈显著负相关。对于 14 种蛋白质，我们观察到与细胞计数/ml 痰呈正相关。多元回归分析表明，一般来说，只有不到 30%的蛋白质变异性可以用所包括的因素来解释。
痰细胞质量对痰上清液中的一些可溶性生物标志物浓度有显著影响。与中、高细胞质量水平相比，低细胞质量的痰样本显示出更高的液体相蛋白变异性。
