米尔布氏提取物和（加尔特纳）杜纳尔提取物可降低阿霉素对H9c2心肌母细胞的细胞毒性。
Mildbr. and (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts.
作者信息
Moukette Bruno M, Castelão-Baptista José P, Ferreira Luciana, Silva Ana M, Simões Rui F, Cabral Célia, Pieme Constant A, Ngogang Jeanne Y, Sardão Vilma A, Oliveira Paulo J
机构信息
Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon.
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, UC Biotech Building, Lot 8A, Biocant Park, Cantanhede 3060-197, Portugal.
出版信息
Evid Based Complement Alternat Med. 2021 Feb 22;2021:8858165. doi: 10.1155/2021/8858165. eCollection 2021.
MATERIALS AND METHODS
Bark extracts of these plants (1 and 25 g/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 M) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts.
RESULTS
The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts significantly inhibited Dox cytotoxicity, with more significant results at the higher concentration. Measurements of selenium and vitamin C in the extracts revealed higher concentration of both when compared with other Cameroonian spices.
CONCLUSION
Both extracts of and were effective against Dox-induced cytotoxicity, most likely due to their content in antioxidants.
材料与方法
在将H9c2心肌母细胞与阿霉素（0.5和1μM）共同孵育24小时之前3小时，添加这些植物的树皮提取物（1和25 g/mL）。在用提取物和/或阿霉素处理后，我们测量了细胞质量和代谢活力、线粒体跨膜电位、超氧阴离子含量以及半胱天冬酶-3和半胱天冬酶-9样活性。此外，还测量了植物提取物中的硒和维生素C含量。
结果
结果证实，阿霉素处理降低了细胞质量、线粒体膜电位和代谢活力，增加了线粒体超氧阴离子，并刺激了半胱天冬酶-3和半胱天冬酶-9样活性。用植物提取物对细胞进行预处理可显著抑制阿霉素的细胞毒性，在较高浓度时效果更显著。提取物中硒和维生素C的测量结果显示，与其他喀麦隆香料相比，两者的浓度都更高。
结论
两种植物的提取物均对阿霉素诱导的细胞毒性有效，最可能是由于其抗氧化剂含量。
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