College of Food Science, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
J Agric Food Chem. 2021 Mar 24;69(11):3312-3325. doi: 10.1021/acs.jafc.0c06815. Epub 2021 Mar 10.
polyphenols (AMPs) can alleviate the degree of liver diseases in rats. However, the mechanism by which this is achieved through gut microbiota modulation remains unclear. Here, a rich-polyphenol extract of (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats. To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR). After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum. The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced. These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases.
多酚（AMPs）可以减轻大鼠肝脏疾病的严重程度。然而，其通过肠道微生物群调节来实现这一目标的机制尚不清楚。在这里，使用富含多酚的提取物来治疗脂多糖（LPS）诱导的大鼠肝脏疾病。为了深入了解抗 LPS 诱导的肝脏疾病，通过 Western blot 分析、免疫组织化学和 16S rRNA 测序或定量实时聚合酶链反应（qRT-PCR）来确定肝功能指标、凋亡蛋白表达、炎症因子和炎症信号通路的激活。在 AMPs 治疗后，肠道微生物群落的组成发生了变化，通过增加肠上皮细胞紧密连接蛋白的表达来促进肠道屏障功能，从而减少血清中的 LPS 含量。炎症因子白细胞介素 6 (IL-6)、白细胞介素 1β (IL-1β)、肿瘤坏死因子α (TNF-α) 和相关 mRNAs 的表达水平降低。这些结果表明，AMPs 作为一种生物活性物质，可以增强肠道屏障功能并调节 LPS 诱导的肝脏疾病的肠道微生物群。
