Department of Chemistry, National Tsing Hua University, 101 Section 2, Kuang Fu Road, Hsinchu 30013, Taiwan.
Chem Commun (Camb). 2021 Apr 11;57(28):3468-3471. doi: 10.1039/d1cc00653c. Epub 2021 Mar 10.
Herein, we applied PmST1 (a sialyltransferase) to achieve acceptor-mediated regioselective sialylation (AMRS) on the nonreducing end GalNH or GalAz (2-azido-2-deoxy galactose). Thus, C5 and C8-modified sialic acid was efficiently assembled on GalNH (or GalAz) to achieve the synthesis of the GAA-7 (one of the echinodermatous gangliosides with higher neuritogenic activity) glycan moiety.
在此，我们应用 PmST1（唾液酸转移酶）在非还原端 GalNH 或 GalAz（2-叠氮-2-脱氧半乳糖）上实现受体质控的区域选择性唾液酸化（AMRS）。因此，C5 和 C8 修饰的唾液酸可有效地在 GalNH（或 GalAz）上组装，从而实现具有更高神经突生成活性的棘皮动物神经节苷脂之一 GAA-7 的聚糖部分的合成。
