• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

CqsA/LuxS-HapR 群体感应电路调节. 中的 VI 型分泌系统 VflT6SS2

CqsA/LuxS-HapR Quorum sensing circuit modulates type VI secretion system VflT6SS2 in .

机构信息

State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, People's Republic of China.

出版信息

Emerg Microbes Infect. 2021 Dec;10(1):589-601. doi: 10.1080/22221751.2021.1902244.

DOI:10.1080/22221751.2021.1902244
PMID:33689580
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8018390/
Abstract

is an emerging enteric pathogen of increasing public health threat. Two quorum sensing (QS) systems, VfqI-VfqR and CqsA/LuxS-HapR, and two type VI secretion systems (T6SSs), VflT6SS1 and VflT6SS2, have been identified in . Whether there exists any correlation between the two systems is unclear. In this study, we found that CqsA/LuxS-HapR circuit regulator LuxO represses while HapR activates VflT6SS2. The effect of LuxO is more pronounced at low cell density and is HapR-dependent. Deletion of abolished Hcp expression and alleviated antibacterial virulence. However, these effects were rescued by HapR-expressing plasmid. Reporter fusion analyses showed that HapR is required for the promoter activities of VflT6SS2. Sequence inspection of the major cluster promoter revealed two potential Motif 1 HapR binding sites, and their bindings to HapR were confirmed by both electrophoretic mobility shift assay (EMSA) and DNase I footprinting assay. Meanwhile, two single Motif 2 sites were identified in 2_a (A) and 2_b (B) promoter regions of the orphan cluster which are less conserved and displayed lower affinities to HapR. Together, our study demonstrated that CqsA/LuxS-HapR QS manipulate VflT6SS2 in , and this finding will enhance our understanding of possible crosstalk between T6SS and QS in microbes.

摘要

是一种新兴的肠道病原体，对公共健康构成越来越大的威胁。已经鉴定出两种群体感应（QS）系统，VfqI-VfqR 和 CqsA/LuxS-HapR，以及两种类型的 VI 型分泌系统（T6SSs），VflT6SS1 和 VflT6SS2。两种系统之间是否存在任何相关性尚不清楚。在本研究中，我们发现 CqsA/LuxS-HapR 电路调节 LuxO 抑制而 HapR 激活 VflT6SS2。在低细胞密度下，LuxO 的作用更为明显，且依赖于 HapR。缺失 会导致 Hcp 表达减少并减轻抗菌毒力。然而，这些效应可以通过表达 HapR 的质粒来挽救。报告基因融合分析表明，HapR 是 VflT6SS2 启动子活性所必需的。主要簇启动子的序列检查显示了两个潜在的 Motif 1 HapR 结合位点，并且通过电泳迁移率变动分析（EMSA）和 DNase I 足迹分析证实了它们与 HapR 的结合。同时，在孤儿簇的 2_a（A）和 2_b（B）启动子区域中鉴定出两个单 Motif 2 位点，这些位点的保守性较低，与 HapR 的亲和力较低。总之，我们的研究表明，CqsA/LuxS-HapR QS 在 中操纵 VflT6SS2，这一发现将增强我们对微生物中 T6SS 和 QS 之间可能发生的串扰的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/5f249c5ac879/TEMI_A_1902244_F0007_OB.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/7170f1dd67de/TEMI_A_1902244_F0001_OB.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/6526bbb0eef9/TEMI_A_1902244_F0002_OB.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/f7788dcf971a/TEMI_A_1902244_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/6379b47a160f/TEMI_A_1902244_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/ed6c85c6bf3e/TEMI_A_1902244_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/5c6fe956778e/TEMI_A_1902244_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/5f249c5ac879/TEMI_A_1902244_F0007_OB.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/7170f1dd67de/TEMI_A_1902244_F0001_OB.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/6526bbb0eef9/TEMI_A_1902244_F0002_OB.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/f7788dcf971a/TEMI_A_1902244_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/6379b47a160f/TEMI_A_1902244_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/ed6c85c6bf3e/TEMI_A_1902244_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/5c6fe956778e/TEMI_A_1902244_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d47/8018390/5f249c5ac879/TEMI_A_1902244_F0007_OB.jpg

相似文献

1
CqsA/LuxS-HapR Quorum sensing circuit modulates type VI secretion system VflT6SS2 in .CqsA/LuxS-HapR 群体感应电路调节. 中的 VI 型分泌系统 VflT6SS2
Emerg Microbes Infect. 2021 Dec;10(1):589-601. doi: 10.1080/22221751.2021.1902244.
2
VfqI-VfqR quorum sensing circuit modulates type VI secretion system VflT6SS2 in .VfqI-VfqR群体感应电路调节VI型分泌系统VflT6SS2在……中（原文此处不完整）
Biochem Biophys Rep. 2022 Jun 2;31:101282. doi: 10.1016/j.bbrep.2022.101282. eCollection 2022 Sep.
3
Characterization of quorum regulatory small RNAs in an emerging pathogen and their roles toward type VI secretion system VflT6SS2 modulation.新兴病原体中群体感应调控小 RNA 的特征及其对 VI 型分泌系统 VflT6SS2 调节的作用。
Emerg Microbes Infect. 2024 Dec;13(1):2396872. doi: 10.1080/22221751.2024.2396872. Epub 2024 Sep 30.
4
[Regulation mechanism of the quorum sensing regulator AphA on the type Ⅵ secretion system VflT6SS2 in ].群体感应调节因子AphA对[中Ⅵ型分泌系统VflT6SS2的调控机制]
Zhonghua Liu Xing Bing Xue Za Zhi. 2024 Apr 10;45(4):566-573. doi: 10.3760/cma.j.cn112338-20231215-00354.
5
Integration Host Factor Modulates the Expression and Function of T6SS2 in .整合宿主因子调节……中T6SS2的表达和功能。 （原文结尾处不完整）
Front Microbiol. 2018 May 15;9:962. doi: 10.3389/fmicb.2018.00962. eCollection 2018.
6
VqsA, a Novel LysR-Type Transcriptional Regulator, Coordinates Quorum Sensing (QS) and Is Controlled by QS To Regulate Virulence in the Pathogen Vibrio alginolyticus.VqsA，一种新型的 LysR 型转录调节因子，协调群体感应（QS）并受 QS 控制，以调节病原菌弧菌的毒力。
Appl Environ Microbiol. 2018 May 31;84(12). doi: 10.1128/AEM.00444-18. Print 2018 Jun 15.
7
CqsA-introduced quorum sensing inhibits type VI secretion system 2 through an OpaR-dependent pathway in Vibrio parahaemolyticus.CqsA 引入的群体感应通过一种依赖 OpaR 的途径抑制副溶血性弧菌的 VI 型分泌系统 2。
Microb Pathog. 2022 Jan;162:105334. doi: 10.1016/j.micpath.2021.105334. Epub 2021 Dec 13.
8
Quorum sensing regulatory cascades control Vibrio fluvialis pathogenesis.群体感应调控级联反应控制弗氏弧菌的发病机制。
J Bacteriol. 2013 Aug;195(16):3583-9. doi: 10.1128/JB.00508-13. Epub 2013 Jun 7.
9
vgrG is separately transcribed from hcp in T6SS orphan clusters and is under the regulation of IHF and HapR.vgrG 是从 T6SS 孤儿簇中的 hcp 中单独转录的，受 IHF 和 HapR 的调控。
Biochem Biophys Res Commun. 2021 Jun 25;559:15-20. doi: 10.1016/j.bbrc.2021.04.092. Epub 2021 Apr 28.
10
Regulation of virulence gene expression in Vibrio cholerae by quorum sensing: HapR functions at the aphA promoter.群体感应调控霍乱弧菌毒力基因表达：HapR在aphA启动子处发挥作用。
Mol Microbiol. 2002 Nov;46(4):1135-47. doi: 10.1046/j.1365-2958.2002.03229.x.

引用本文的文献

1
Evaluation of the Multivalent Immunoprotective Effects of Protein, DNA, and IgY Vaccines Against Outer Membrane Protein VF14355 in .蛋白质、DNA和卵黄抗体（IgY）疫苗针对[具体对象]中外膜蛋白VF14355的多价免疫保护作用评估
Int J Mol Sci. 2025 Apr 4;26(7):3379. doi: 10.3390/ijms26073379.
2
Quorum sensing in Vibrio controls carbon metabolism to optimize growth in changing environmental conditions.群体感应控制弧菌的碳代谢以优化在不断变化的环境条件下的生长。
PLoS Biol. 2024 Nov 11;22(11):e3002891. doi: 10.1371/journal.pbio.3002891. eCollection 2024 Nov.
3
Characterization of quorum regulatory small RNAs in an emerging pathogen and their roles toward type VI secretion system VflT6SS2 modulation.

本文引用的文献

1
Time-resolved dual RNA-seq of tissue uncovers Pseudomonas plecoglossicida key virulence genes in host-pathogen interaction with Epinephelus coioides.组织的时间分辨双 RNA-seq 揭示了假单胞菌 plecoglossicida 在与 Epinephelus coioides 的宿主-病原体相互作用中的关键毒力基因。
Environ Microbiol. 2020 Feb;22(2):677-693. doi: 10.1111/1462-2920.14884. Epub 2019 Dec 14.
2
Structure and Activity of the Type VI Secretion System.VI 型分泌系统的结构与活性
Microbiol Spectr. 2019 Jul;7(4). doi: 10.1128/microbiolspec.PSIB-0031-2019.
3
Emergence of Azithromycin Resistance Mediated by Phosphotransferase-Encoding (A) in Diarrheagenic Vibrio fluvialis.
新兴病原体中群体感应调控小 RNA 的特征及其对 VI 型分泌系统 VflT6SS2 调节的作用。
Emerg Microbes Infect. 2024 Dec;13(1):2396872. doi: 10.1080/22221751.2024.2396872. Epub 2024 Sep 30.
4
Virulence, antibiotic resistance phenotypes and molecular characterisation of Vibrio furnissii isolates from patients with diarrhoea.从腹泻患者中分离的弗氏弧菌的毒力、抗生素耐药表型和分子特征。
BMC Infect Dis. 2024 Apr 19;24(1):412. doi: 10.1186/s12879-024-09273-5.
5
Transcriptome profiling of type VI secretion system core gene mutant of highlights regulators controlling diverse functions ranging from virulence to metabolism.转录组谱分析表明，VI 型分泌系统核心基因 突变体调控多种功能，包括毒力和代谢。
Microbiol Spectr. 2024 Jan 11;12(1):e0285223. doi: 10.1128/spectrum.02852-23. Epub 2023 Nov 29.
6
Quorum sensing inhibits interference competition among bacterial symbionts within a host.群体感应抑制了宿主内细菌共生体之间的干扰竞争。
Curr Biol. 2023 Oct 9;33(19):4244-4251.e4. doi: 10.1016/j.cub.2023.08.051. Epub 2023 Sep 8.
7
Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level.VI 型分泌系统在转录、转录后和翻译后水平的调控。
Microbiology (Reading). 2023 Aug;169(8). doi: 10.1099/mic.0.001376.
8
TssI2-TsiI2 of VflT6SS2 delivers pesticin domain-containing periplasmic toxin and cognate immunity that modulates bacterial competitiveness.VflT6SS2 的 TssI2-TsiI2 输送含有杀虫素结构域的周质毒素和同源免疫蛋白，从而调节细菌竞争力。
Gut Microbes. 2022 Jan-Dec;14(1):2136460. doi: 10.1080/19490976.2022.2136460.
9
A Quorum Sensing-Regulated Type VI Secretion System Containing Multiple Nonredundant VgrG Proteins Is Required for Interbacterial Competition in Chromobacterium violaceum.群体感应调控的包含多种非冗余 VgrG 蛋白的 VI 型分泌系统是 Chromobacterium violaceum 种间竞争所必需的。
Microbiol Spectr. 2022 Aug 31;10(4):e0157622. doi: 10.1128/spectrum.01576-22. Epub 2022 Jul 25.
10
VfqI-VfqR quorum sensing circuit modulates type VI secretion system VflT6SS2 in .VfqI-VfqR群体感应电路调节VI型分泌系统VflT6SS2在……中（原文此处不完整）
Biochem Biophys Rep. 2022 Jun 2;31:101282. doi: 10.1016/j.bbrep.2022.101282. eCollection 2022 Sep.
腹泻性弗氏弧菌中磷转移酶编码（A）介导的阿奇霉素耐药性的出现。
mSphere. 2019 Jun 12;4(3):e00215-19. doi: 10.1128/mSphere.00215-19.
4
Integration Host Factor Modulates the Expression and Function of T6SS2 in .整合宿主因子调节……中T6SS2的表达和功能。 （原文结尾处不完整）
Front Microbiol. 2018 May 15;9:962. doi: 10.3389/fmicb.2018.00962. eCollection 2018.
5
A Highly Promiscuous Integron, Plasmids, Extended Spectrum Beta Lactamases and Efflux Pumps as Factors Governing Multidrug Resistance in a Highly Drug Resistant Isolate BD146 from Kolkata, India.一种高度混杂的整合子、质粒、超广谱β-内酰胺酶及外排泵作为印度加尔各答一株高度耐药菌株BD146中多药耐药性的影响因素
Indian J Microbiol. 2018 Mar;58(1):60-67. doi: 10.1007/s12088-017-0687-8. Epub 2017 Oct 31.
6
Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticus.丝氨酸/苏氨酸激酶 PpkA 协调海洋病原体弧菌在 T6SS2 激活和群体感应之间的相互作用。
Environ Microbiol. 2018 Feb;20(2):903-919. doi: 10.1111/1462-2920.14039. Epub 2018 Jan 19.
7
The Type VI Secretion System: A Dynamic System for Bacterial Communication?VI型分泌系统：一种用于细菌交流的动态系统？
Front Microbiol. 2017 Jul 28;8:1454. doi: 10.3389/fmicb.2017.01454. eCollection 2017.
8
Functional Characterization and Conditional Regulation of the Type VI Secretion System in .. 中VI型分泌系统的功能表征与条件调控
Front Microbiol. 2017 Mar 30;8:528. doi: 10.3389/fmicb.2017.00528. eCollection 2017.
9
Transcriptional regulation of cpsQ-mfpABC and mfpABC by CalR in Vibrio parahaemolyticus.副溶血性弧菌中CalR对cpsQ-mfpABC和mfpABC的转录调控
Microbiologyopen. 2017 Aug;6(4). doi: 10.1002/mbo3.470. Epub 2017 Mar 20.
10
Rules of Engagement: The Type VI Secretion System in Vibrio cholerae.作战规则：霍乱弧菌中的VI型分泌系统
Trends Microbiol. 2017 Apr;25(4):267-279. doi: 10.1016/j.tim.2016.12.003. Epub 2016 Dec 24.