Appl Opt. 2021 Mar 10;60(8):2150-2157. doi: 10.1364/AO.415684.
Saccharomyces cerevisiae(S. cerevisiae) has been classically used as a treatment for diarrhea and diarrhea-related diseases. However, cases of the fungal infections caused by S. cerevisiae have been increasing in the last two decades among immunocompromised patients, while a long time was spent on S. cerevisiae isolation clinically so it was difficult to achieve timely diagnosis the diseases. Here, a novel approach for isolation and selection of S. cerevisiae is proposed by designing a microfluidic chip with an optically induced dielectrophoresis (ODEP) system. S. cerevisiae was isolated from the surroundings by ODEP due to different dielectrophoretic forces. Two special light images were designed and used to block and separate S. cerevisiae, respectively, and several manipulation parameters of ODEP were experimentally optimized to acquire the maximum isolation efficiency of S. cerevisiae. The results on the S. cerevisiae isolation declared that the purity of the S. cerevisiae selected by the method was up to 99.5±0.05, and the capture efficiency was up to 65.0±2.5 within 10 min. This work provides a general method to isolate S. cerevisiae as well as other microbial cells with high accuracy and efficiency and paves a road for biological research in which the isolation of high-purity cells is required.
酿酒酵母（Saccharomyces cerevisiae）一直被经典地用于治疗腹泻和腹泻相关疾病。然而，在过去的二十年中，免疫功能低下的患者中由酿酒酵母引起的真菌感染病例一直在增加，而临床上花费了很长时间来分离酿酒酵母，因此很难及时诊断这些疾病。在这里，通过设计具有光诱导介电泳（ODEP）系统的微流控芯片，提出了一种分离和选择酿酒酵母的新方法。由于不同的介电泳力，酿酒酵母通过 ODEP 从周围环境中分离出来。设计了两个特殊的光图像来分别阻挡和分离酿酒酵母，并通过实验优化了 ODEP 的几个操作参数，以获得酿酒酵母的最大分离效率。酿酒酵母的分离结果表明，该方法选择的酿酒酵母的纯度高达 99.5±0.05，并且在 10 分钟内的捕获效率高达 65.0±2.5。这项工作为分离酿酒酵母以及其他具有高精度和高效率的微生物细胞提供了一种通用方法，为需要高纯度细胞分离的生物研究铺平了道路。
