Du Mingao, Liu Fei, Luan Xiaoli, Li Gongxin
Key Laboratory of Advanced Process Control for Light Industry (Ministry of Education), Institute of Automation, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China.
Biomed Opt Express. 2022 Jan 4;13(2):559-570. doi: 10.1364/BOE.448729. eCollection 2022 Feb 1.
has been classically used to treat diarrhea and diarrhea-related diseases. However, in the past two decades, fungal infections caused by have been increasing among immunocompromised patients, and it takes too long to isolate from blood to diagnose it in time. In this paper, a new method for the isolation and selection of from red blood cells (RBC) is proposed by designing a microfluidic chip with an optically-induced dielectrophoresis (ODEP) system. It was verified by theory and experiments that the magnitude and direction of the dielectrophoresis force applied on RBCs and are different, which determine that the can be isolated from RBCs by the ODEP system. By designing the specific light images and the dynamic separation mode, the optimal operating conditions were experimentally achieved for acquiring higher purity of . The purity ranges were up to 95.9%-97.3%. This work demonstrates a promising tool for efficient and effective purification of from RBCs and provides a novel method of isolation for the timely diagnosis of fungal infections.
传统上一直用于治疗腹泻及与腹泻相关的疾病。然而，在过去二十年中，由[具体真菌名称未给出]引起的真菌感染在免疫功能低下的患者中不断增加，而且从血液中分离出[具体真菌名称未给出]以及时进行诊断所需时间过长。在本文中，通过设计一种带有光诱导介电泳（ODEP）系统的微流控芯片，提出了一种从红细胞（RBC）中分离和筛选[具体真菌名称未给出]的新方法。理论和实验均验证了施加在红细胞和[具体真菌名称未给出]上的介电泳力的大小和方向不同，这决定了可以通过ODEP系统从红细胞中分离出[具体真菌名称未给出]。通过设计特定的光图像和动态分离模式，通过实验实现了获取更高纯度[具体真菌名称未给出]的最佳操作条件。纯度范围高达95.9% - 97.3%。这项工作展示了一种从红细胞中高效纯化[具体真菌名称未给出]的有前景的工具，并为真菌感染的及时诊断提供了一种新型的[具体真菌名称未给出]分离方法。
