一种基于香豆素的“开-关”型荧光探针，用于高选择性检测硫化氢并在活细胞中成像。
A coumarin-based "off-on" fluorescent probe for highly selective detection of hydrogen sulfide and imaging in living cells.
作者信息
Ou Luying, Guo Rui, Lin Weiying
机构信息
Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, P. R. China.
出版信息
Anal Methods. 2021 Mar 28;13(12):1511-1516. doi: 10.1039/d1ay00097g. Epub 2021 Mar 10.
Hydrogen sulfide (HS), as a significant signaling molecule, is associated with diverse physiological and pathological processes. However, it's still a challenge to explore outstanding tools for detecting endogenous HS in vivo. Thus, a simple "off-on" HS fluorescent probe CMHS has been reasonably designed, and it is based on coumarin as the fluorophore group. The probe CMHS displayed a crucial turn-on fluorescence enhancement (180-fold), rapid reaction time, high selectivity, and a low limit of detection (2.31 × 10 M). Additionally, probe CMHS could be applied to visualize exogenous and endogenous HS successfully in HeLa cells with low cytotoxicity and good permeability.
硫化氢（HS）作为一种重要的信号分子，与多种生理和病理过程相关。然而，探索用于体内检测内源性HS的优秀工具仍然是一项挑战。因此，合理设计了一种基于香豆素作为荧光基团的简单“开-关”型HS荧光探针CMHS。探针CMHS表现出关键的荧光开启增强（180倍）、快速的反应时间、高选择性和低检测限（2.31×10⁻⁶M）。此外，探针CMHS可成功应用于HeLa细胞中外源性和内源性HS的可视化，具有低细胞毒性和良好的通透性。
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