Accotto G P, Donson J, Mullineaux P M
John Innes Institute, AFRC Institute for Plant Science Research, Norwich, UK.
EMBO J. 1989 Apr;8(4):1033-9. doi: 10.1002/j.1460-2075.1989.tb03470.x.
All, except 19 [corrected] bp, of the Digitaria streak virus (DSV) genome is transcribed. Two RNA transcripts (1+ and 2+) are encoded by the virion DNA strand and up to five (1- to 5-) by the complementary DNA strand [corrected]. Detailed mapping of these RNAs has revealed evidence for splicing in one species (RNA 4-), which together with its more abundant unspliced counterpart (RNA 2-) could synthesize both a 30.5 and 41 kd polypeptide from the same transcription unit. This extensive overlapping of spliced and unspliced RNAs could indicate that the initiation and splicing of transcripts is temporally regulated. At least one transcript (RNA 1-) may have a non-translational role. Transcription of the DSV genome shows similarities to some animal DNA viruses, particularly the papovaviruses.
除19个[校正后]碱基对以外，马唐线条病毒（DSV）基因组的其余部分均被转录。病毒粒子DNA链编码两条RNA转录本（1+和2+），互补DNA链则编码多达五条（1-至5-）[校正后]。对这些RNA的详细图谱分析显示，有一种RNA（RNA 4-）存在剪接证据，它与其更丰富的未剪接对应物（RNA 2-）一起，能够从同一转录单元合成30.5和41千道尔顿的多肽。剪接和未剪接RNA的这种广泛重叠可能表明转录本的起始和剪接受到时间调控。至少有一种转录本（RNA 1-）可能具有非翻译作用。DSV基因组的转录与某些动物DNA病毒，特别是乳头瘤病毒，存在相似之处。
