Il'kevich Iu G, Lemeshko N N, Mar'iankova R F, Lukashevich I S, Klimenko S M
Vopr Virusol. 1988 Jan-Feb;33(1):75-81.
Ultrafiltration through hollow fibrous filters followed by purification in interrupted and linear urografin gradients yielded a Lassa virus suspension of high concentration. The use of gamma-irradiation for inactivation of the frozen virus suspension (-70 degrees C) caused no apparent structural changes of virions and made it possible to examine Lassa virus in electron microscope by negative staining. The observed virus particles in their morphology and sizes did not differ from previously described particles of other members of the Arenaviridae family. In ultrathin sections of Lassa virus-infected Vero cells, atypical virions were sometimes visible alongside with typical particles. Within one type of such particles no ribosome-like granules could be detected. Such "hollow" particles may possibly be defective virions. Another kind of atypical particles contained homogeneous electron-dense core and resembled mycoplasma. Of greatest interest are the particles with heterogeneous core in which "sandy" granules can be distinguished. The presence of greater amounts of uranophilic material than usually may be explained by getting into the virion in the process of its formation of a greater amount of genetic material than that present in typical virions.
通过中空纤维过滤器进行超滤，然后在间断和线性泛影葡胺梯度中进行纯化，得到了高浓度的拉沙病毒悬液。使用γ射线辐照灭活冷冻病毒悬液（-70℃）并未导致病毒粒子出现明显的结构变化，这使得通过负染色在电子显微镜下检查拉沙病毒成为可能。观察到的病毒粒子在形态和大小上与先前描述的沙粒病毒科其他成员的粒子并无差异。在感染拉沙病毒的Vero细胞超薄切片中，有时除了典型粒子外还可见非典型病毒粒子。在其中一种此类粒子中未检测到核糖体样颗粒。这种“空心”粒子可能是有缺陷的病毒粒子。另一种非典型粒子含有均匀的电子致密核心，类似支原体。最令人感兴趣的是具有异质核心的粒子，其中可以区分出“沙质”颗粒。嗜铀物质的量比通常更多，这可能是由于在病毒粒子形成过程中比典型病毒粒子摄取了更多的遗传物质所致。
