Elliott L H, McCormick J B, Johnson K M
J Clin Microbiol. 1982 Oct;16(4):704-8. doi: 10.1128/jcm.16.4.704-708.1982.
Because of the cumbersome conditions experienced in a maximum containment laboratory, methods for inactivating highly pathogenic viruses were investigated. The infectivity of Lassa, Marburg, and Ebola viruses was inactivated without altering the immunological activity after radiation with Co60 gamma rays. At 4 degrees C, Lassa virus was the most difficult to inactivate with a rate of 5.3 X 10(-6) log 50% tissue culture infective dose per rad of CO60 radiation, as compared with 6.8 X 10(-6) log 50% tissue culture infective dose per rad for Ebola virus and 8.4 X 10(-6) log 50% tissue culture infective dose per rad for Marburg virus. Experimental inactivation curves, as well as curves giving the total radiation needed to inactivate a given concentration of any of the three viruses, are presented. We found this method of inactivation to be superior to UV light or beta-propiolactone inactivation and now routinely use it for preparation of material for protein-chemistry studies or for preparation of immunological reagents.
由于在最高级别的生物安全实验室中进行操作存在诸多不便，因此对高致病性病毒的灭活方法展开了研究。在用钴60伽马射线进行辐射后，拉沙病毒、马尔堡病毒和埃博拉病毒的传染性被灭活，且免疫活性未受影响。在4摄氏度的条件下，拉沙病毒最难被灭活，其灭活速率为每拉德钴60辐射5.3×10⁻⁶ log 50%组织培养感染剂量，相比之下，埃博拉病毒为每拉德6.8×10⁻⁶ log 50%组织培养感染剂量，马尔堡病毒为每拉德8.4×10⁻⁶ log 50%组织培养感染剂量。文中给出了实验性灭活曲线，以及灭活给定浓度这三种病毒中任何一种所需的总辐射量曲线。我们发现这种灭活方法优于紫外线或β-丙内酯灭活法，目前已将其常规用于蛋白质化学研究材料的制备或免疫试剂的制备。
