Lukashevich I S, Mar'iankova R F, Fidarov F M
Vopr Virusol. 1981 Jul-Aug(4):452-6.
Acute and persistent infections of Vero cells with a cloned Lassa virus were studied. After acute infection at a multiplicity of 2 PFU/cell the latent period was 12-16 hrs and the maximum yield of virus particles in the growth medium was obtained 28-32 hours postinfection. A persistently infected cell line was established by inoculating Vero cells with the cloned Lassa virus followed by subcultivation of the infected cells. The infected cells underwent 36 passages. Lassa virus production by the infected cells followed a cyclic pattern varying from 10(2) to 10(5) PFU/ml. Neither the plating efficiency of the virus nor its growth curves changes at 35 degrees C and 39 degrees C. The replication of the cloned virus in infected cells (superinfection) was markedly depressed in comparison to that of normal Vero cells infected with Lassa virus. The role of defective interfering particles in the establishment of persistent infection is discussed.
对克隆的拉沙病毒感染非洲绿猴肾细胞（Vero细胞）的急性和持续性感染进行了研究。以每细胞2个蚀斑形成单位（PFU）的感染复数进行急性感染后，潜伏期为12 - 16小时，感染后28 - 32小时在生长培养基中获得病毒颗粒的最大产量。通过用克隆的拉沙病毒接种Vero细胞，然后对感染细胞进行传代培养，建立了持续感染的细胞系。感染细胞传代了36次。感染细胞产生拉沙病毒呈周期性模式，范围从10²到10⁵ PFU/毫升。在35摄氏度和39摄氏度时，病毒的铺板效率及其生长曲线均未改变。与感染拉沙病毒的正常Vero细胞相比，克隆病毒在感染细胞中的复制（超感染）明显受到抑制。讨论了缺陷干扰颗粒在建立持续感染中的作用。
