Department of Horticulture and Biotechnology, Chinese Culture University, Taipei, 11114, Taiwan.
Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, 700, Taiwan.
Plant Sci. 2021 Apr;305:110849. doi: 10.1016/j.plantsci.2021.110849. Epub 2021 Feb 16.
IbACP, Ipomoea batatas anti-cancer peptide, a sixteen-amino-acid peptide isolated from sweet potato leaves, is capable of mediating a rapid alkalinization of growth media in plant suspension cells. However, the biological roles of IbACP as a defense peptide have not been studied. The objective of this study was to investigate the effect of IbACP on the accumulation of reactive oxygen species (ROS) and the expression of the defense-related genes. IbACP treatment of sweet potato leaves resulted in marked accumulation of both superoxide ion (O) and hydrogen peroxide (HO). The activity of peroxidase (POD) was significantly enhanced by IbACP treatment, suggesting that high levels of POD antioxidant activity may be used to scavenge the excess HO in sweet potato plants. The IbACP-related genes were identified by suppression subtractive hybridization (SSH), and were then classified and assigned to the following categories: defense, development, metabolism, signaling, gene expression, and abiotic stress. HO acts as a second messenger for gene activation in some of the IbACP-triggered gene expressions. These results demonstrated that IbACP is part of an integrated strategy for genetic regulation in sweet potato. Our work highlights the function of IbACP and its potential use for enhancing stress tolerance in sweet potato, in an effort to improve our understanding of defense-response mechanisms.
IbACP，即甘薯叶抗癌肽，是从甘薯叶中分离得到的一种十六肽，能够介导植物悬浮细胞生长培养基的快速碱化。然而，IbACP 作为防御肽的生物学作用尚未得到研究。本研究旨在探讨 IbACP 对活性氧（ROS）积累和防御相关基因表达的影响。IbACP 处理甘薯叶导致超氧阴离子（O）和过氧化氢（HO）的明显积累。过氧化物酶（POD）的活性因 IbACP 处理而显著增强，表明高水平的 POD 抗氧化活性可用于清除甘薯植物中过量的 HO。通过抑制差减杂交（SSH）鉴定与 IbACP 相关的基因，然后将其分类并分配到以下类别：防御、发育、代谢、信号转导、基因表达和非生物胁迫。HO 作为一些 IbACP 触发的基因表达中基因激活的第二信使。这些结果表明，IbACP 是甘薯遗传调控综合策略的一部分。我们的工作强调了 IbACP 的功能及其在提高甘薯耐胁迫性方面的潜在用途，旨在增进我们对防御反应机制的理解。
