树脂基水门汀对带血运牙本质/牙髓组织工程模拟物的生物相容性评估。
Biocompatibility assessment of resin-based cements on vascularized dentin/pulp tissue-engineered analogues.
机构信息
Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki (AUTh), Thessaloniki, GR-54124, Greece.
Department of Materials Science and Technology, University of Crete, Heraklion, Greece; Institute of Electronic Structure and Laser, Foundation for Research and Technology Hellas (FORTH), Heraklion, Greece.
出版信息
Dent Mater. 2021 May;37(5):914-927. doi: 10.1016/j.dental.2021.02.019. Epub 2021 Mar 7.
OBJECTIVES
A three-dimensional (3D) dentin/pulp tissue analogue, resembling the human natural tissue has been engineered in an in vitro setup, aiming to assess the cytocompatibility of resin-based dental restorative cements.
METHODS
Stem Cells from Apical Papilla (SCAP) and Human Umbilical Vein Endothelial Cells (HUVEC) were embedded in Collagen-I/Fibrin hydrogels at 1:3 ratio within 24-well plates. Hanging culture inserts were placed over the hydrogels, housing an odontoblast-like cell layer and a human treated-dentin barrier. Shear modulus of the hydrogels at 3.5 and 5 mg/ml was evaluated by dynamic mechanical analysis. Eluates of two resin-based cements, a dual-cure- (Breeze™, Pentron: Cement-1/C1), and a self-adhesive cement (SpeedCEMplus™, Ivoclar-Vivadent: Cement-2/C2) were applied into the dentin/pulp tissue analogue after pre-stimulation with LPS. Cytocompatibility was assessed by MTT assay, live/dead staining and real-time PCR analysis.
RESULTS
Both hydrogel concentrations showed similar shear moduli to the natural pulp until day (D) 7, while the 5 mg/ml-hydrogel substantially increased stiffness by D14. Both cements caused no significant toxicity to the dentin/pulp tissue analogue. C1 induced stimulation (p < 0.01) of cell viability (158 ± 3%, 72 h), while pre-stimulation with LPS attenuated this effect. C2 (±LPS) caused minor reduction of viability (15-20%, 24 h) that recovered at 72 h for the LPS+ group. Both cements caused upregulation of VEGF, ANGP-1, and downregulation of the respective receptors VEGFR-2 and Tie-1.
SIGNIFICANCE
Both resin-based cements showed good cytocompatibility and triggered angiogenic response within the dentin/pulp tissue analogue, indicating initiation of pulp repair responses to the released xenobiotics.
目的
构建了一种三维（3D）牙本质/牙髓组织模拟物，类似于人体天然组织，旨在评估基于树脂的牙科修复水泥的细胞相容性。
方法
将根尖乳头干细胞（SCAP）和人脐静脉内皮细胞（HUVEC）以 1:3 的比例嵌入胶原-I/纤维蛋白水凝胶中，放置在 24 孔板中。将悬挂培养插入物放置在水凝胶上方，形成牙本质样细胞层和人处理牙本质屏障。通过动态力学分析评估水凝胶在 3.5 和 5 mg/ml 时的剪切模量。在用 LPS 预处理后，将两种基于树脂的水泥（双固化型（Breeze™，Pentron：水泥 1/C1）和自粘接型水泥（SpeedCEMplus™，Ivoclar-Vivadent：水泥 2/C2）的洗脱液应用于牙本质/牙髓组织模拟物中。通过 MTT 测定、死活染色和实时 PCR 分析评估细胞相容性。
结果
两种水凝胶浓度在第 7 天之前与天然牙髓具有相似的剪切模量，而 5mg/ml 水凝胶在第 14 天显著增加了刚度。两种水泥对牙本质/牙髓组织模拟物均无明显毒性。C1 诱导细胞活力（158±3%，72 h）刺激（p<0.01），而 LPS 预处理减弱了这种作用。C2（±LPS）导致活力轻微降低（24 h 时为 15-20%），LPS+组在 72 h 时恢复。两种水泥均导致 VEGF、ANGP-1 的上调和相应受体 VEGFR-2 和 Tie-1 的下调。
意义
两种基于树脂的水泥均表现出良好的细胞相容性，并在牙本质/牙髓组织模拟物中引发血管生成反应，表明对释放的异源物启动了牙髓修复反应。
Zotero 插件