School of Light Industry, Beijing Technology and Business University.
China Rural Technology Development Center.
J Oleo Sci. 2021 Apr 2;70(4):571-580. doi: 10.5650/jos.ess20274. Epub 2021 Mar 10.
Polyglycerol monolaurates are generally recognized as safe food additives and are commonly used as food emulsifiers. In this study, the antimicrobial effect of four polyglycerol monolaurates on two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (Escherichia. coli and Pseudomonas aeruginosa) were investigated. The minimum inhibitory concentration (MIC) of diglycerol monolaurate (PG2ML), triglycerol monolaurate (PG3ML), hexaglycerol monolaurate (PG6ML), and decaglycerol monolaurate (PG10ML) against S. aureus was 0.16, 0.32, 0.63, and 1.25 mg/mL, respectively. The MIC of PG2ML, PG3ML, PG6ML, and PG10ML against B. subtilis was 0.32, 0.63, 1.25, and 3.75 mg/mL, respectively. No apparent antimicrobial effect of these four polyglycerol monolaurates on E. coli and P. aeruginosa was observed even up to 10.00 mg/mL. The underlying mechanism was investigated by assessing cell membrane permeability, the integrity of cell membrane, and morphology. We concluded that polyglycerol monolaurates might eliminate Gram-positive bacteria by disrupting the cell membrane, thereby increasing cell membrane permeability, releasing the cellular contents, and altering the cell morphology.
聚甘油单月桂酸酯通常被认为是安全的食品添加剂，常用于食品乳化剂。本研究考察了四种聚甘油单月桂酸酯（二甘醇单月桂酸酯（PG2ML）、三甘醇单月桂酸酯（PG3ML）、六甘醇单月桂酸酯（PG6ML）和十甘醇单月桂酸酯（PG10ML））对两种革兰氏阳性菌（金黄色葡萄球菌和枯草芽孢杆菌）和两种革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）的抗菌效果。PG2ML、PG3ML、PG6ML 和 PG10ML 对金黄色葡萄球菌的最小抑菌浓度（MIC）分别为 0.16、0.32、0.63 和 1.25mg/mL。PG2ML、PG3ML、PG6ML 和 PG10ML 对枯草芽孢杆菌的 MIC 分别为 0.32、0.63、1.25 和 3.75mg/mL。即使浓度高达 10.00mg/mL，这四种聚甘油单月桂酸酯对大肠杆菌和铜绿假单胞菌也没有明显的抗菌作用。通过评估细胞膜通透性、细胞膜完整性和形态学，研究了其潜在的作用机制。我们得出结论，聚甘油单月桂酸酯可能通过破坏细胞膜来消除革兰氏阳性菌，从而增加细胞膜通透性，释放细胞内容物，并改变细胞形态。
