从琼脂糖凝胶中同时进行DNA片段的电洗脱和浓缩。

Simultaneous electroelution and concentration of DNA fragments from agarose gels.

作者信息

Stroop W G

机构信息

Neurovirology Research Laboratory, Veterans Administration Medical Center, Salt Lake City, Utah 84148.

出版信息

Anal Biochem. 1988 Feb 15;169(1):194-6. doi: 10.1016/0003-2697(88)90273-4.

DOI:10.1016/0003-2697(88)90273-4

PMID:3369681

Abstract

A rapid and inexpensive method for the electroelution of DNA fragments from agarose gels is described. DNA fragments were separated by agarose gel electrophoresis and visualized by staining with ethidium bromide. Selected DNA fragments were placed into electroeluter tubes capped with dialysis membrane and electroeluted into a small volume of buffer using a conventional horizontal gel electrophoresis apparatus. The method successfully eluted and concentrated DNA fragments with molecular weights ranging from 2.7 to 13.9 MDa in 3 h.

摘要

本文描述了一种从琼脂糖凝胶中电洗脱DNA片段的快速且廉价的方法。DNA片段通过琼脂糖凝胶电泳进行分离，并用溴化乙锭染色进行可视化。将选定的DNA片段放入用透析膜封口的电洗脱管中，使用传统的水平凝胶电泳装置将其电洗脱到少量缓冲液中。该方法在3小时内成功洗脱并浓缩了分子量范围为2.7至13.9 MDa的DNA片段。