从聚丙烯酰胺凝胶和琼脂糖凝胶中电洗脱DNA和蛋白质。

Electroelution of DNA and protein from polyacrylamide and agarose gels.

作者信息

Chang T, Tsai I H, Chang W C

机构信息

Institute of Biochemical Sciences, National Taiwan University, Taipei, Republic of China.

出版信息

Biochem Int. 1987 Oct;15(4):687-91.

PMID:3435534

Abstract

An electroelution method is described for the recovery of DNA and protein from agarose or polyacrylamide gels. The samples to be electroeluted are compartmentalized in a modified microcentrifuge tube fitted with dialysis membranes. This procedure is simple, rapid, inexpensive and efficient. Within 30 min to 2 hrs, the recovery of the sample is nearly quantitative. DNA fragments recovered can be directly subjected to DNA sequence analysis or enzymatic reactions after ethanol precipitation. Proteins can also be recovered after separation by acrylamide gel in the presence or absence of detergents and be ready for further analysis.

摘要

本文描述了一种从琼脂糖或聚丙烯酰胺凝胶中回收DNA和蛋白质的电洗脱方法。待电洗脱的样品被分隔在装有透析膜的改良微量离心管中。该方法简单、快速、廉价且高效。在30分钟至2小时内，样品回收率接近定量。回收的DNA片段在乙醇沉淀后可直接进行DNA序列分析或酶促反应。蛋白质也可在有或没有去污剂存在的情况下通过丙烯酰胺凝胶分离后回收，并准备进行进一步分析。