Carlá V, Lombardi G, Beni M, Russi P, Moneti G, Moroni F
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Anal Biochem. 1988 Feb 15;169(1):89-94. doi: 10.1016/0003-2697(88)90258-8.
Kynurenic acid, a biologically active tryptophan metabolite, has been identified and measured in the rat brain and other organs using HPLC and GC/MS. Both the described methods required extraction of the compound in alkaline ethanol and initial purification on Dowex ion-exchange resins. The GC/MS approach used 3-hydroxy-2-naphthoic acid as an internal standard and a derivatization procedure with diazomethane and trifluoroacetic anhydride. The HPLC procedure was performed on a reverse-phase column using a spectrophotometric detector. Both the GC/MS and the HPLC methods had the lowest detection limit in the range of 10 pmol/injection, but the variability of the results was lower when HPLC was used. HPLC analysis showed the content of kynurenic acid to be 14 +/- 2 pmol/g wet wt in the brain, 75 +/- 7 in the heart, 87 +/- 8 in the liver, and 298 +/- 10 in the kidneys. Comparable but variable values were obtained with GC/MS.
犬尿喹啉酸是一种具有生物活性的色氨酸代谢产物，已通过高效液相色谱法（HPLC）和气相色谱-质谱联用法（GC/MS）在大鼠大脑及其他器官中得到鉴定和测定。上述两种方法均需在碱性乙醇中提取该化合物，并首先在Dowex离子交换树脂上进行纯化。GC/MS方法使用3-羟基-2-萘甲酸作为内标，并采用了与重氮甲烷和三氟乙酸酐的衍生化程序。HPLC程序是在反相柱上使用分光光度检测器进行的。GC/MS和HPLC方法的最低检测限均在10皮摩尔/进样范围内，但使用HPLC时结果的变异性较低。HPLC分析显示，犬尿喹啉酸的含量在大脑中为14±2皮摩尔/克湿重，在心脏中为75±7，在肝脏中为87±8，在肾脏中为298±10。使用GC/MS获得了可比但有差异的值。
